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水稻黄斑驳病毒抗性基因RYMV3的精细定位:一个来自光稃稻的水稻黄斑驳病毒新抗性基因

Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima.

作者信息

Pidon Hélène, Ghesquière Alain, Chéron Sophie, Issaka Souley, Hébrard Eugénie, Sabot François, Kolade Olufisayo, Silué Drissa, Albar Laurence

机构信息

Plant Diversity Adaptation and Development Research Unit, Institut de Recherche pour le Développement - Université de Montpellier, Montpellier, France.

Africa Rice Center, Cotonou, Benin.

出版信息

Theor Appl Genet. 2017 Apr;130(4):807-818. doi: 10.1007/s00122-017-2853-0. Epub 2017 Jan 31.

Abstract

A new resistance gene against Rice yellow mottle virus was identified and mapped in a 15-kb interval. The best candidate is a CC-NBS-LRR gene. Rice yellow mottle virus (RYMV) disease is a serious constraint to the cultivation of rice in Africa and selection for resistance is considered to be the most effective management strategy. The aim of this study was to characterize the resistance of Tog5307, a highly resistant accession belonging to the African cultivated rice species (Oryza glaberrima), that has none of the previously identified resistance genes to RYMV. The specificity of Tog5307 resistance was analyzed using 18 RYMV isolates. While three of them were able to infect Tog5307 very rapidly, resistance against the others was effective despite infection events attributed to resistance-breakdown or incomplete penetrance of the resistance. Segregation of resistance in an interspecific backcross population derived from a cross between Tog5307 and the susceptible Oryza sativa variety IR64 showed that resistance is dominant and is controlled by a single gene, named RYMV3. RYMV3 was mapped in an approximately 15-kb interval in which two candidate genes, coding for a putative transmembrane protein and a CC-NBS-LRR domain-containing protein, were annotated. Sequencing revealed non-synonymous polymorphisms between Tog5307 and the O. glaberrima susceptible accession CG14 in both candidate genes. An additional resistant O. glaberrima accession, Tog5672, was found to have the Tog5307 genotype for the CC-NBS-LRR gene but not for the putative transmembrane protein gene. Analysis of the cosegregation of Tog5672 resistance with the RYMV3 locus suggests that RYMV3 is also involved in Tog5672 resistance, thereby supporting the CC-NBS-LRR gene as the best candidate for RYMV3.

摘要

一个新的抗水稻黄斑驳病毒基因被鉴定出来,并定位在一个15千碱基对的区间内。最佳候选基因是一个CC-NBS-LRR基因。水稻黄斑驳病毒(RYMV)病严重制约了非洲水稻的种植,选育抗性品种被认为是最有效的管理策略。本研究的目的是对Tog5307的抗性进行表征,Tog5307是非洲栽培稻种(光稃稻)中的一个高抗材料,它没有先前鉴定出的对RYMV的抗性基因。使用18个RYMV分离株分析了Tog5307抗性的特异性。其中三个分离株能够非常迅速地感染Tog5307,尽管存在因抗性丧失或抗性不完全显性导致的感染情况,但对其他分离株的抗性仍然有效。Tog5307与感病的亚洲栽培稻品种IR64杂交产生的种间回交群体中抗性的分离表明,抗性是显性的,由一个名为RYMV3的单基因控制。RYMV3被定位在一个约15千碱基对的区间内,该区间注释了两个候选基因,分别编码一个假定的跨膜蛋白和一个含CC-NBS-LRR结构域的蛋白。测序揭示了两个候选基因在Tog5307和光稃稻感病材料CG14之间存在非同义多态性。另一个光稃稻抗性材料Tog5672被发现CC-NBS-LRR基因具有Tog5307基因型,但假定的跨膜蛋白基因不具有该基因型。Tog5672抗性与RYMV3位点的共分离分析表明,RYMV3也参与了Tog5672的抗性,从而支持CC-NBS-LRR基因作为RYMV3的最佳候选基因。

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