用于风湿性心脏病的基因特异性阻抗细菌DNA传感器
Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease.
作者信息
Singh Swati, Kaushal Ankur, Gupta Sunil, Kumar Ashok
机构信息
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025 India.
出版信息
Indian J Microbiol. 2017 Mar;57(1):112-115. doi: 10.1007/s12088-016-0620-6. Epub 2016 Sep 22.
An impedimetric gene specific DNA sensor was developed by immobilization of single stranded DNA probe onto the screen printed modified gold-dendrimer nanohybrid composite electrode for early and rapid detection of in human throat swab samples causing rheumatic heart disease. Electrochemical impedance response was measured after hybridization with bacterial single stranded genomic DNA (ssG-DNA) with probe. The sensor was found highly specific to and can detect as low as 0.01 ng ssDNA in 6 µL sample only in 30 min. The nanohybrid sensor was also tested with non-specific pathogens and characterized by FTIR. An early detection of the pathogen in human can save damage of mitral and aortic heart valves (rheumatic heart disease) by proper medical care.
通过将单链DNA探针固定在丝网印刷修饰的金-树枝状大分子纳米杂化复合电极上，开发了一种阻抗式基因特异性DNA传感器，用于早期快速检测导致风湿性心脏病的人类咽拭子样本中的[病原体名称未给出]。与细菌单链基因组DNA（ssG-DNA）与探针杂交后，测量电化学阻抗响应。发现该传感器对[病原体名称未给出]具有高度特异性，仅在30分钟内就能在6微升样本中检测低至0.01纳克的ssDNA。该纳米杂化传感器还对非特异性病原体进行了测试，并通过傅里叶变换红外光谱（FTIR）进行了表征。早期检测人类中的病原体[病原体名称未给出]，通过适当的医疗护理可以避免二尖瓣和主动脉心脏瓣膜受损（风湿性心脏病）。
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