LMO4是一种参与非小细胞肺癌细胞迁移和侵袭的预后标志物。
LMO4 is a prognostic marker involved in cell migration and invasion in non-small-cell lung cancer.
作者信息
Wang Wenjun, Wu Sipei, Guo Minzhang, He Jianxing
机构信息
State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
Lung Cancer Research Institute and Cancer Center, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
出版信息
J Thorac Dis. 2016 Dec;8(12):3682-3690. doi: 10.21037/jtd.2016.12.22.
Abstract
BACKGROUND
The aims of this study were to analyze the association of LMO4 with non-small-cell lung cancer (NSCLC) survival rate, and to determine its functional role and signaling pathway in lung cancer.
METHODS
Immunohistochemistry (IHC) was used to detect the expression of LMO4 in NSCLC cell lines and tumor tissues. Migration and invasion ability was detected respectively by wound healing test and transwell test. Immunofluorescence and western blot were detected of AKT/PI3K pathway related genes MAPK, PI3K, AKT.
RESULTS
LMO4 has high expression level of NSCLC cell lines and tumor tissues, and correlated with a lower survival rate. LMO4 can regulate the migration and invasion of NSCLC cells through the AKT/PI3K pathway.
CONCLUSIONS
LMO4 could serve as a promising biomarker and therapeutic target for NSCLC.
摘要
背景
本研究旨在分析LMO4与非小细胞肺癌（NSCLC）生存率的相关性，并确定其在肺癌中的功能作用和信号通路。
方法
采用免疫组织化学（IHC）检测LMO4在NSCLC细胞系和肿瘤组织中的表达。分别通过伤口愈合试验和Transwell试验检测迁移和侵袭能力。检测AKT/PI3K通路相关基因MAPK、PI3K、AKT的免疫荧光和蛋白质印迹。
结果
LMO4在NSCLC细胞系和肿瘤组织中表达水平较高，且与较低的生存率相关。LMO4可通过AKT/PI3K通路调节NSCLC细胞的迁移和侵袭。
结论
LMO4有望成为NSCLC的生物标志物和治疗靶点。
相似文献
1
LMO4 is a prognostic marker involved in cell migration and invasion in non-small-cell lung cancer.
J Thorac Dis. 2016 Dec;8(12):3682-3690. doi: 10.21037/jtd.2016.12.22.
2
Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer.
Cell Physiol Biochem. 2017;42(4):1431-1446. doi: 10.1159/000479207. Epub 2017 Jul 17.
3
MicroRNA-92a promotes epithelial-mesenchymal transition through activation of PTEN/PI3K/AKT signaling pathway in non-small cell lung cancer metastasis.
Int J Oncol. 2017 Jul;51(1):235-244. doi: 10.3892/ijo.2017.3999. Epub 2017 May 16.
4
MiR-4299 suppresses non-small cell lung cancer cell proliferation, migration and invasion through modulating PTEN/AKT/PI3K pathway.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(11):3408-3414. doi: 10.26355/eurrev_201806_15163.
5
LMO4 promotes the invasion and proliferation of gastric cancer by activating PI3K-Akt-mTOR signaling.
Am J Transl Res. 2019 Oct 15;11(10):6534-6543. eCollection 2019.
6
LncRNA MALAT1 promotes migration and invasion of non-small-cell lung cancer by targeting miR-206 and activating Akt/mTOR signaling.
Anticancer Drugs. 2018 Sep;29(8):725-735. doi: 10.1097/CAD.0000000000000650.
7
MiR-598 Suppresses Invasion and Migration by Negative Regulation of Derlin-1 and Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer.
Cell Physiol Biochem. 2018;47(1):245-256. doi: 10.1159/000489803. Epub 2018 May 11.
8
IL-17A/IL-17RA promotes invasion and activates MMP-2 and MMP-9 expression via p38 MAPK signaling pathway in non-small cell lung cancer.
Mol Cell Biochem. 2019 May;455(1-2):195-206. doi: 10.1007/s11010-018-3483-9. Epub 2018 Dec 18.
9
The KLF6 splice variant KLF6-SV1 promotes proliferation and invasion of non-small cell lung cancer by up-regultating PI3K-AKT signaling pathway.
J Cancer. 2019 Aug 29;10(22):5324-5331. doi: 10.7150/jca.34212. eCollection 2019.
10
Alpha-enolase promotes cell glycolysis, growth, migration, and invasion in non-small cell lung cancer through FAK-mediated PI3K/AKT pathway.
J Hematol Oncol. 2015 Mar 8;8:22. doi: 10.1186/s13045-015-0117-5.
引用本文的文献
1
LOC100506691 Is an Oncogenic Long Noncoding RNA that Promotes Growth and Motility of Lung Cancer Cells.
Cancer Genomics Proteomics. 2025 Sep-Oct;22(5):775-790. doi: 10.21873/cgp.20536.
2
Nimodipine Used with Vincristine: Protects Schwann Cells and Neuronal Cells from Vincristine-Induced Cell Death but Increases Tumor Cell Susceptibility.
Int J Mol Sci. 2024 Sep 27;25(19):10389. doi: 10.3390/ijms251910389.
3
KRAS and TP53 co-mutation predicts benefit of immune checkpoint blockade in lung adenocarcinoma.
Br J Cancer. 2024 Aug;131(3):524-533. doi: 10.1038/s41416-024-02746-z. Epub 2024 Jun 12.
4
A Bayesian method to infer copy number clones from single-cell RNA and ATAC sequencing.
PLoS Comput Biol. 2023 Nov 2;19(11):e1011557. doi: 10.1371/journal.pcbi.1011557. eCollection 2023 Nov.
5
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles carrying miR-655-3p inhibit the development of esophageal cancer by regulating the expression of HIF-1α via a LMO4/HDAC2-dependent mechanism.
Cell Biol Toxicol. 2023 Aug;39(4):1319-1339. doi: 10.1007/s10565-022-09759-5. Epub 2022 Oct 12.
6
Single-Molecule RNA Sequencing Reveals IFNγ-Induced Differential Expression of Immune Escape Genes in Merkel Cell Polyomavirus-Positive MCC Cell Lines.
Front Microbiol. 2021 Dec 22;12:785662. doi: 10.3389/fmicb.2021.785662. eCollection 2021.
7
CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis.
Dis Markers. 2021 Dec 9;2021:3977189. doi: 10.1155/2021/3977189. eCollection 2021.
8
FOXM1 Promotes Head and Neck Squamous Cell Carcinoma Activation of the Linc-ROR/LMO4/AKT/PI3K Axis.
Front Oncol. 2021 Aug 10;11:658712. doi: 10.3389/fonc.2021.658712. eCollection 2021.
9
Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-Related Penile Squamous Cell Carcinoma.
Am J Mens Health. 2021 Mar-Apr;15(2):15579883211011380. doi: 10.1177/15579883211011380.
10
Circ_0058124 Aggravates the Progression of Papillary Thyroid Carcinoma by Activating LMO4 Expression via Targeting miR-370-3p.
Cancer Manag Res. 2020 Oct 1;12:9459-9470. doi: 10.2147/CMAR.S271778. eCollection 2020.
本文引用的文献
1
The LIM-domain only protein 4 contributes to lung epithelial cell proliferation but is not essential for tumor progression.
Respir Res. 2015 Jun 7;16(1):67. doi: 10.1186/s12931-015-0228-0.
2
CD147-CD98hc complex contributes to poor prognosis of non-small cell lung cancer patients through promoting cell proliferation via the PI3K/Akt signaling pathway.
Ann Surg Oncol. 2014 Dec;21(13):4359-68. doi: 10.1245/s10434-014-3816-1. Epub 2014 Aug 2.
3
Roles of PI3K/Akt and c-Jun signaling pathways in human papillomavirus type 16 oncoprotein-induced HIF-1α, VEGF, and IL-8 expression and in vitro angiogenesis in non-small cell lung cancer cells.
PLoS One. 2014 Jul 24;9(7):e103440. doi: 10.1371/journal.pone.0103440. eCollection 2014.
4
Structural basis of the interaction of the breast cancer oncogene LMO4 with the tumour suppressor CtIP/RBBP8.
J Mol Biol. 2013 Apr 12;425(7):1101-10. doi: 10.1016/j.jmb.2013.01.017. Epub 2013 Jan 23.
5
The oncoprotein HBXIP activates transcriptional coregulatory protein LMO4 via Sp1 to promote proliferation of breast cancer cells.
Carcinogenesis. 2013 Apr;34(4):927-35. doi: 10.1093/carcin/bgs399. Epub 2013 Jan 5.
6
LMO4 inhibits p53-mediated proliferative inhibition of breast cancer cells through interacting p53.
Life Sci. 2012 Sep 24;91(9-10):358-63. doi: 10.1016/j.lfs.2012.08.005. Epub 2012 Aug 11.
7
LMO4 expression in squamous cell carcinoma of the anterior tongue.
Histopathology. 2011 Feb;58(3):477-80. doi: 10.1111/j.1365-2559.2011.03765.x.
8
Repression of Lim only protein 4-activated transcription inhibits proliferation and induces apoptosis of normal mammary epithelial cells and breast cancer cells.
Clin Exp Metastasis. 2010 Oct;27(7):455-63. doi: 10.1007/s10585-010-9332-1. Epub 2010 Jun 6.
9
Tumor and host factors that may limit efficacy of chemotherapy in non-small cell and small cell lung cancer.
Crit Rev Oncol Hematol. 2010 Sep;75(3):173-234. doi: 10.1016/j.critrevonc.2009.11.006. Epub 2010 Jan 4.
10
Inhibition of Akt activity induces the mesenchymal-to-epithelial reverting transition with restoring E-cadherin expression in KB and KOSCC-25B oral squamous cell carcinoma cells.
J Exp Clin Cancer Res. 2009 Feb 26;28(1):28. doi: 10.1186/1756-9966-28-28.
Zotero 插件