一种检测核DNA G-四链体的红色近红外荧光染料：体外分析与细胞成像
A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging.
作者信息
Doria F, Nadai M, Zuffo M, Perrone R, Freccero M, Richter S N
机构信息
Dept. of Chemistry, University of Pavia, V.le Taramelli 10, 27100 Pavia, Italy.
Dept. of Molecular Medicine, University of Padua, via Gabelli 63, 35121 Padua, Italy.
出版信息
Chem Commun (Camb). 2017 Feb 14;53(14):2268-2271. doi: 10.1039/c6cc08492c.
Abstract
Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleolin displacement reveal that the compound targets and thus makes visible nuclear DNA G-quadruplexes.
摘要
对于一种原型核心扩展萘二酰亚胺，研究了其在核酸G-四链体结合时的聚集、红-近红外发射和点亮特性，该化合物能够快速进入细胞并定位于核仁。与抗G-四链体抗体的高度共定位以及核仁素置换均表明，该化合物靶向并因此使核DNA G-四链体可见。
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