生物样品中β-鼠胆酸分析的副产物，以三乙酸甲酯形式存在。
By-products in the analysis of beta-muricholic acid in biological samples as methyl ester triacetate.
作者信息
Janssen G, Toppet S, Compernolle F, Parmentier G
机构信息
Rega Institute, University of Leuven, Belgium.
出版信息
Steroids. 1989 Jun;53(6):677-93. doi: 10.1016/0039-128x(89)90060-3.
By-products were formed on analysis of beta-muricholic acid (3 alpha, 6 beta, 7 beta-trihydroxy-5 beta-cholan-24-oic acid) in biological samples by a method involving acid-catalyzed solvolysis of sulfate esters in acetone-methanol, followed by perchloric acid-catalyzed acetylation with acetic anhydride-acetic acid. These products have been identified by mass spectrometry and nuclear magnetic resonance as methyl 3-0,6-0-diacetyl-7-0-(1-methyl-3-oxo-1-butenyl)- and methyl 3-0,7-0-diacetyl-6-0-(1-methyl-3-oxo-1-butenyl)-beta-muricholate, methyl 3-0, 6-0-diacetyl- and methyl 3-0, 7-0-diacetyl-beta-muricholate, and a methyl diacetoxy-cholen-24-oate.
在通过一种方法分析生物样品中的β-鼠胆酸（3α，6β，7β-三羟基-5β-胆烷-24-酸）时形成了副产物，该方法包括在丙酮-甲醇中对硫酸酯进行酸催化溶剂解，然后用乙酸酐-乙酸进行高氯酸催化的乙酰化。这些产物已通过质谱和核磁共振鉴定为3-O，6-O-二乙酰基-7-O-（1-甲基-3-氧代-1-丁烯基）-和3-O，7-O-二乙酰基-6-O-（1-甲基-3-氧代-1-丁烯基）-β-鼠胆酸甲酯、3-O，6-O-二乙酰基-β-鼠胆酸甲酯和3-O，7-O-二乙酰基-β-鼠胆酸甲酯，以及一种二乙酰氧基胆烷-24-酸甲酯。
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