Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
Beijing Key Laboratory of Radioactive Waste Treatment, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China.
Talanta. 2017 Apr 1;165:182-187. doi: 10.1016/j.talanta.2016.12.054. Epub 2016 Dec 22.
We developed a unique continuously evolving colorimetric sensor array based on AuNPs decorated by two single-stranded oligonucleotides with different molar ratios for protein discrimination. The number of differential receptors in this sensor array could be easily extended by adjusting the molar ratios of two DNA, resulting in continuously improved discrimination ability. The continuous response data of target samples against our sensing system could be easily obtained and exclude abnormal signals. The sensing system could discriminate twelve proteins at the concentration of 200nM in the presence of 50% human urine with accuracy of 100%, showing feasible potential for diagnostic applications. Remarkably, HSA at various concentrations, the pure Lys and HSA, and the mixture of these two proteins with different molar ratios had been successfully discriminated in LDA plot as well in the presence of human urine sample. This novel strategy will be very promising for the design of cheaper and more reliable sensor arrays for target samples.
我们开发了一种独特的连续进化比色传感器阵列，基于 AuNPs 修饰的两种具有不同摩尔比的单链寡核苷酸，用于蛋白质鉴别。通过调整两种 DNA 的摩尔比，可以轻松扩展此传感器阵列中的差分受体数量，从而不断提高鉴别能力。可以轻松获得针对我们传感系统的目标样品的连续响应数据，并排除异常信号。该传感系统在存在 50%人尿的情况下，能够以 100%的准确率鉴别 200nM 浓度的十二种蛋白质，显示出在诊断应用中的可行潜力。值得注意的是，在存在人尿样品的情况下，各种浓度的 HSA、纯 Lys 和 HSA 以及这两种蛋白质以不同摩尔比的混合物在 LDA 图中也得到了成功的区分。这种新策略对于设计更便宜、更可靠的目标样品传感器阵列将非常有前景。
