聚（苯乙烯-co-DPHA-co-EDMA）整体柱的制备及其在 HPLC 中对小分子和生物大分子的分离应用。
Preparation of a poly(styrene-co-DPHA-co-EDMA) monolith and its application for the separation of small molecules and biomacromolecules by HPLC.
机构信息
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
出版信息
Talanta. 2017 Apr 1;165:339-345. doi: 10.1016/j.talanta.2016.12.071. Epub 2016 Dec 27.
A high performance liquid chromatography (HPLC) monolithic column was prepared by redox polymerization of styrene, dipentaerythritol hexaacrylate (DPHA) and ethylene glycol dimethacrylate (EDMA) in a porogen system of n-propanol/PEG400. The monolith was characterized by scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP) and the results indicated that the monolith had a stable and homogeneous structure. The porosity of the monolithic column was 75.86% and average pore diameter was 2.1µm. Several alkylbenzenes and anilines were used to evaluate the column performance in terms of hydrophobicity. Then the column was applied to separate small molecules including phytosterol and BSA tryptic digest. Finally, five standard proteins, egg white and plasma were separated respectively and high separation capacity of protein was obtained.
一种高效液相色谱（HPLC）整体柱通过在 n-丙醇/聚乙二醇 400 的孔形成剂体系中聚合苯乙烯、二季戊四醇六丙烯酸酯（DPHA）和乙二醇二甲基丙烯酸酯（EDMA）制备而成。整体柱通过扫描电子显微镜（SEM）、压汞孔隙率（MIP）进行了表征，结果表明整体柱具有稳定均一的结构。整体柱的孔隙率为 75.86%，平均孔径为 2.1µm。使用几种烷基苯和苯胺来评估柱的疏水性。然后，该柱被用于分离小分子，包括植物甾醇和 BSA 酶解物。最后，分别分离了 5 种标准蛋白质、蛋清和血浆，并获得了高的蛋白质分离能力。
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