State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Talanta. 2017 Apr 1;165:577-583. doi: 10.1016/j.talanta.2017.01.019. Epub 2017 Jan 7.
A simple, efficient and versatile biosensing platform capable of the multiplexed detection for glucose, lactate and choline was developed by the integration of bipolar electrochemistry and electrochemiluminescence (ECL) imaging. The sensing bipolar electrodes (BPEs) were simply modified via a one-step method adaptable to different enzymes. The biorecognition event happening between the substrate and the corresponding enzyme could be directly reported by the ECL emitted on the same pole from luminol and in situ generated HO. Under optimized conditions, the BPEs array was successfully applied for the determination of glucose, lactate and choline in the ranges of 0.01-1mM, 0.01-1mM and 0.02-5mM, with the LOD of 7.57μM, 8.25μM and 43.19μM, respectively. Owing to the improved stability of in situ generated HO, a whole series of analytes testing could be completed in the same BPE biochip. Subsequently, an array chip consisting of nine BPEs enabled the concomitant detection of glucose, lactate and choline, demonstrating the capability for multifunctional detection of biomolecules. This versatile analytical system could be easily extended to sensitive screening in a miniaturized device and point of care testing.
通过将双极电化学和电致化学发光（ECL）成像集成，开发了一种简单、高效且多功能的生物传感平台，能够用于葡萄糖、乳酸和胆碱的多重检测。通过适用于不同酶的一步法，简单地修饰了传感双极电极（BPE）。在优化条件下，成功地将 BPE 阵列应用于在 0.01-1mM、0.01-1mM 和 0.02-5mM 范围内测定葡萄糖、乳酸和胆碱，其检测限分别为 7.57μM、8.25μM 和 43.19μM。由于原位生成的 HO 的稳定性得到提高，因此可以在同一 BPE 生物芯片上完成一系列分析物的测试。随后，由九个 BPE 组成的阵列芯片能够同时检测葡萄糖、乳酸和胆碱，证明了用于生物分子多功能检测的能力。这种多功能分析系统可以很容易地扩展到小型化设备中的敏感筛选和现场检测。
