三肽基氨肽酶I的组织化学显示

Histochemical Demonstration of Tripeptidyl Aminopeptidase I.

作者信息

Dimitrova Mashenka B, Atanasova Dimitrinka Y, Lazarov Nikolai E

机构信息

Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.

Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

出版信息

Methods Mol Biol. 2017;1560:55-68. doi: 10.1007/978-1-4939-6788-9_4.

DOI:10.1007/978-1-4939-6788-9_4

PMID:28155145

Abstract

Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI-glycyl-L-prolyl-L-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-L-prolyl-L-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs.

摘要

酶组织化学方法对于研究酶参与不同病理过程具有重要价值。在此，我们描述两种用于显色和荧光组织化学显示三肽基氨基肽酶I（TPPI）的方案，TPPI是一种对神经元功能至关重要的蛋白酶。这些程序基于新合成的TPPI底物——甘氨酰-L-脯氨酰-L-甲硫氨酰-5-氯-1-蒽醌酰肼（GPM-CAH）和甘氨酰-L-脯氨酰-L-甲硫氨酰-4-肼基-N-己基-1,8-萘二甲酰亚胺（GPM-HHNI）。使用这些方案，可以在哺乳动物器官的组织切片中获得精确的酶定位。