Dimitrova Mashenka B, Atanasova Dimitrinka Y, Lazarov Nikolai E
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Methods Mol Biol. 2017;1560:55-68. doi: 10.1007/978-1-4939-6788-9_4.
Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI-glycyl-L-prolyl-L-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-L-prolyl-L-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs.
酶组织化学方法对于研究酶参与不同病理过程具有重要价值。在此，我们描述两种用于显色和荧光组织化学显示三肽基氨基肽酶I（TPPI）的方案，TPPI是一种对神经元功能至关重要的蛋白酶。这些程序基于新合成的TPPI底物——甘氨酰-L-脯氨酰-L-甲硫氨酰-5-氯-1-蒽醌酰肼（GPM-CAH）和甘氨酰-L-脯氨酰-L-甲硫氨酰-4-肼基-N-己基-1,8-萘二甲酰亚胺（GPM-HHNI）。使用这些方案，可以在哺乳动物器官的组织切片中获得精确的酶定位。
