Department of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine Trakia, University of Stara Zagora, Bulgaria.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Science, Sofia, Bulgaria.
Pol J Vet Sci. 2021 Sep;24(3):409-414. doi: 10.24425/pjvs.2021.138732.
Distribution of tripeptidyl peptidase I (TPPI) activity in the structures of porcine lumbar spinal ganglia (LSG) was studied by enzyme histochemistry on cryostat sections from all the ganglia using the substrate glycyl-L-prolyl-L-methionyl-5-chloro-1-anthraquinonyl hydrazide (GPM-CAH) and 4-nitrobenzaldehyde (NBA) as visualization factor. Light microscopic observations showed TPPI activity in almost all the LSG structures. The enzyme reaction in different cell types was compared semi-quantitatively. Strong reaction was observed in the small neurons, satellite ganglia cells and some nerve fibers. Weak reactivity was found in the large sensory somatic neurons, whereas moderate reaction for TPPI was determined in the middle sensory somatic neurons and some nerve fibers. Statistical analysis by one-way ANOVA showed no significance of difference (when p⟨0.05) for the number of TPPI positive neurons per mm2. The original data obtained by the enzyme histochemistry method give us a reason to presume that TPPI actively participates in the functions of all the neuronal structures in porcine LSG. According to our results, it could be suggested that TPPI activity is important for the functions of autonomic and somatic sensory neurons.
猪腰脊神经节（LSG）中三肽基肽酶 I（TPPI）活性的分布通过使用甘氨酰-L-脯氨酰-L-甲硫氨酰-5-氯-1-蒽醌基酰肼（GPM-CAH）和 4-硝基苯甲醛（NBA）作为可视化因子的冷冻切片酶组织化学在所有神经节结构中进行了研究。光镜观察显示，TPPI 活性存在于 LSG 的几乎所有结构中。对不同细胞类型的酶反应进行了半定量比较。在小神经元、卫星神经节细胞和一些神经纤维中观察到强烈的反应。在大感觉躯体神经元中发现了较弱的反应性，而在中感觉躯体神经元和一些神经纤维中则确定了 TPPI 的中等反应性。单因素方差分析的统计分析显示，每平方毫米的 TPPI 阳性神经元数量没有差异（当 p ⟨ 0.05 时）。通过酶组织化学方法获得的原始数据使我们有理由假设 TPPI 积极参与猪 LSG 中所有神经元结构的功能。根据我们的结果，可以提出 TPPI 活性对于自主和躯体感觉神经元的功能很重要。
