免疫组织化学中的热诱导抗原修复：机制与应用
Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications.
作者信息
Yamashita Shuji, Katsumata Osamu
机构信息
Department of Pathology, School of Medicine, Keio University, 35-Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Department of Anatomy, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 228-8555, Japan.
出版信息
Methods Mol Biol. 2017;1560:147-161. doi: 10.1007/978-1-4939-6788-9_10.
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter.
热诱导抗原修复（HIAR）是当前免疫组织化学的一项重要技术。我们描述了HIAR的机制及其针对福尔马林固定、石蜡包埋标本和冰冻切片的方案，这些是使用光学显微镜进行免疫组织化学最常用的材料。此外，在本章中，我们还描述了针对高度隐蔽表位的抗原修复方法以及使用辣根过氧化物酶（HRP）标记抗体法和免疫荧光法进行双重免疫染色的方法。
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