Demarco Diego
Laboratório de Anatomia Vegetal, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Sao Paulo, Brazil.
Methods Mol Biol. 2017;1560:313-330. doi: 10.1007/978-1-4939-6788-9_24.
Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion. As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes. Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
组织化学分析对于研究植物分泌结构至关重要，其分类至少部分基于分泌物的组成。由于每个腺体可能产生一种或多种物质，因此应使用各种组织化学测试来检测不同化学类别的代谢物，以对其分泌物进行正确分析。在此，我描述一些在分泌结构中检测碳水化合物、蛋白质、脂质、酚类化合物和生物碱的最常用方法。
