Zhang Lianglin, Zhou Fang, Wang Shuai, Xu Xudong
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
FEBS Lett. 2017 Mar;591(5):751-759. doi: 10.1002/1873-3468.12582. Epub 2017 Feb 20.
Upon N-stepdown, Anabaena sp. PCC 7120 differentiates heterocysts along filaments in a semiregular pattern. A 17-amino acid peptide called PatS is a morphogen precursor for pattern formation. The principal PatS derivative involved in heterocyst patterning has been proposed to be the C-terminal peptide PatS-5 (RGSGR), PatS-6 (ERGSGR), or PatS-8 (CDERGSGR). We present the first evidence for processing of PatS in cell extracts of this cyanobacterium. PatS is probably cleaved between the C-terminal 7th and 8th amino acid residues, producing PatS-7 (DERGSGR), then converted into PatS-6 and PatS-5. The processing site could be changed by a substitution at the C-terminal 8th residue.
在N步下调时，鱼腥藻PCC 7120沿丝状体以半规则模式分化出异形胞。一种名为PatS的17个氨基酸的肽是模式形成的形态发生素前体。有人提出参与异形胞模式形成的主要PatS衍生物是C末端肽PatS-5（RGSGR）、PatS-6（ERGSGR）或PatS-8（CDERGSGR）。我们首次提供了在这种蓝细菌的细胞提取物中加工PatS的证据。PatS可能在C末端第7和第8个氨基酸残基之间被切割，产生PatS-7（DERGSGR），然后转化为PatS-6和PatS-5。加工位点可能会因C末端第8个残基的取代而改变。
