Małaczewska Joanna, Wójcik Roman, Kaczorek Edyta, Rękawek Wojciech, Siwicki Andrzej Krzysztof
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 13, 10-718 Olsztyn, Poland.
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 13, 10-718 Olsztyn, Poland.
Res Vet Sci. 2017 Feb;110:4-11. doi: 10.1016/j.rvsc.2016.10.013. Epub 2016 Oct 24.
Recent intensive development of nanotechnology has broadened the use of noble metal nanocolloids in alternative medicine. Meanwhile, silver and copper nanoparticles are tested as potential feed additives in pig farming. Experiments on rodents prove that metal nanocolloids easily interact with macrophages and lymphocytes, although the specific nature of pigs' immune system means that rodent tests may not reflect fully the responsiveness of porcine T cells. Our purpose was to demonstrate the effect of a commercial gold nanocolloid on percentages and proliferation of T lymphocyte subpopulations and on IL-2 and IL-10 synthesis in porcine peripheral blood mononuclear cells tested in vitro. The nanocolloid was not cytotoxic to porcine leukocytes, nor did it affect resting T cells. However, high nanogold concentrations inhibited proliferation of mitogen-stimulated CD4, CD4CD8α and CD4CD8α T cells by down-regulation of the IL-2 synthesis and increased the percentage of CD4CD8α double negative T cells, probably by depressing their ability to express a CD8α marker after activation. The observations implicate potential immunosuppressive activity of nanogold and strong influence of nanoparticles on CD4CD8α T cells, the most abundant subpopulation in young animals, suggests its particular effect on a developing immune system.
近年来纳米技术的迅猛发展拓宽了贵金属纳米胶体在替代医学中的应用。与此同时，银和铜纳米颗粒作为潜在的饲料添加剂正在养猪业中进行测试。对啮齿动物的实验表明，金属纳米胶体很容易与巨噬细胞和淋巴细胞相互作用，尽管猪免疫系统的特殊性质意味着啮齿动物实验可能无法完全反映猪T细胞的反应性。我们的目的是证明一种商业金纳米胶体对体外测试的猪外周血单个核细胞中T淋巴细胞亚群的百分比和增殖以及IL-2和IL-10合成的影响。该纳米胶体对猪白细胞无细胞毒性，也不影响静息T细胞。然而，高浓度的纳米金通过下调IL-2合成抑制有丝分裂原刺激的CD4、CD4CD8α和CD4CD8α T细胞的增殖，并可能通过抑制其激活后表达CD8α标记的能力增加CD4CD8α双阴性T细胞的百分比。这些观察结果表明纳米金具有潜在的免疫抑制活性，并且纳米颗粒对CD4CD8α T细胞（幼龄动物中最丰富的亚群）有强烈影响，提示其对发育中的免疫系统有特殊作用。
