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Food Chem. 2017 Jun 1;224:280-288. doi: 10.1016/j.foodchem.2016.12.079. Epub 2016 Dec 23.
Avenanthramides (AVNs) are a family of nitrogen-containing phenolic compounds produced in oat; AVN 2c, 2p, and 2f are the three major members. An LC-MS/MS method was developed, with the limit of detection (LOD) and the limit of quantitation (LOQ) being, respectively, 0.29ng/mL and 1.96ng/mL for AVN 2c, 0.24ng/mL and 0.60ng/mL for AVE 2p, and 0.42ng/mL and 2.2ng/mL for AVN 2f. The method was validated in oat-containing hot cereal and snack bar samples. The recovery of AVN 2c, 2p, and 2f from these two oat products was 95-113%, and the relative standard deviations ranged from 5% to 9%. This method was used to evaluate oat products and raw oat samples. The effects of location and variety on AVN composition were investigated. The method presented here provides a novel and rapid tool to quantitate the abundance of AVN 2c, 2p, and 2f in oat-containing products.
燕麦素（AVNs）是一类在燕麦中产生的含氮酚类化合物；AVN2c、2p 和 2f 是三种主要成员。开发了一种 LC-MS/MS 方法，AVN2c 的检测限（LOD）和定量限（LOQ）分别为 0.29ng/mL 和 1.96ng/mL，AVE2p 的 LOD 和 LOQ 分别为 0.24ng/mL 和 0.60ng/mL，AVN2f 的 LOD 和 LOQ 分别为 0.42ng/mL 和 2.2ng/mL。该方法在含有燕麦的热谷类和小吃棒样品中进行了验证。从这两种燕麦产品中回收 AVN2c、2p 和 2f 的回收率为 95-113%，相对标准偏差范围为 5%-9%。该方法用于评估燕麦产品和生燕麦样品。研究了地点和品种对 AVN 组成的影响。本文提出的方法为定量分析含有燕麦的产品中 AVN2c、2p 和 2f 的丰度提供了一种新颖而快速的工具。
