Tomar Priya, Garg Amit, Gupta Rashee, Singh Abhishek, Gupta Navratan Kumar, Upadhyay Amit
Departments of Pediatrics and *Microbiology, LLRM Medical College, Meerut, Uttar Pradesh, India. Correspondence to: Dr Amit Upadhyay, Head, Department of Pediatrics, LLRM Medical college, Meerut, Uttar Pradesh. (India).
Indian Pediatr. 2017 Mar 15;54(3):199-203. doi: 10.1007/s13312-017-1030-5. Epub 2017 Feb 2.
To evaluate efficacy of two blood cultures taken simultaneously from two different sites as compared to standard practice of single blood culture in diagnosis of neonatal sepsis.
Prospective cohort study.
A tertiary-care center at a public hospital.
475 neonates admitted to intensive care unit with suspected sepsis, from August 2014-July 2015.
Two blood cultures drawn from two different peripheral veins in patients with suspected neonatal sepsis.
Increase in culture-positivity rate with use of two blood cultures.
475 babies with suspected sepsis were enrolled. 185 patients had only first culture positive (38.9%). When we added second culture positivity, yield increased to 221 (46.5%). Adding on second culture increased the culture yield by 36 (7.6%; 95% CI 2.41 to 12.79; P=0.018). The most common organisms isolated were E. coli, S. aureus and Candida spp. Major morbidities and mortality were more common in blood culture positive patients Contamination was ruled out in 25 babies who grew Coagulase negative Staphylococcus (CONS) (n=10) and Candida spp. (n=15) in either of the two cultures.
Two blood cultures taken simultaneously from two different sites improve rate of pathogen detection as compared to routine practice of single blood culture.
评估与单份血培养的标准做法相比，同时从两个不同部位采集两份血培养在新生儿败血症诊断中的疗效。
前瞻性队列研究。
一家公立医院的三级医疗中心。
2014年8月至2015年7月入住重症监护病房且疑似败血症的475例新生儿。
对疑似新生儿败血症患者从两条不同的外周静脉采集两份血培养。
使用两份血培养时培养阳性率的增加情况。
纳入475例疑似败血症婴儿。185例患者仅首次培养呈阳性（38.9%）。当加入第二次培养阳性结果时，阳性率增至221例（46.5%）。加入第二次培养使培养阳性率提高了36例（7.6%；95%可信区间2.41至12.79；P = 0.018）。分离出的最常见病原体为大肠杆菌、金黄色葡萄球菌和念珠菌属。血培养阳性患者中主要发病率和死亡率更为常见。在两份培养物中任何一份培养出凝固酶阴性葡萄球菌（CONS）（n = 10）和念珠菌属（n = 15）的25例婴儿中排除了污染。
与单份血培养的常规做法相比，同时从两个不同部位采集两份血培养可提高病原体检测率。
