School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
Bioresour Technol. 2017 Apr;230:49-55. doi: 10.1016/j.biortech.2017.01.033. Epub 2017 Jan 25.
In present study, the feasibility of partial nitrification (PN) process achievement and its greenhouse gas emission were evaluated in a sequencing batch biofilm reactor (SBBR). After 90days' operation, the average effluent NH-N removal efficiency and nitrite accumulation rate of PN-SBBR were high of 98.2% and 87.6%, respectively. Both polysaccharide and protein contents were reduced in loosely bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS) during the achievement of PN-biofilm. Excitation-emission matrix spectra implied that aromatic protein-like, tryptophan protein-like and humic acid-like substances were the main compositions of both kinds of EPS in seed sludge and PN-biofilm. According to typical cycle, the emission rate of CO had a much higher value than that of NO, and their total amounts per cycle were 67.7 and 16.5mg, respectively. Free ammonia (FA) played a significant role on the inhibition activity of nitrite-oxidizing bacteria and the occurrence of nitrite accumulation.
在本研究中，采用序批式生物膜反应器（SBBR）评估了部分硝化（PN）过程的可行性及其温室气体排放。经过 90 天的运行，PN-SBBR 的平均出水 NH4-N 去除效率和亚硝酸盐积累率分别高达 98.2%和 87.6%。在实现 PN 生物膜的过程中，松散结合胞外聚合物（LB-EPS）和紧密结合胞外聚合物（TB-EPS）中的多糖和蛋白质含量均有所降低。激发-发射矩阵光谱表明，芳香族蛋白类、色氨酸蛋白类和腐殖酸类物质是种子污泥和 PN 生物膜中两种 EPS 的主要组成部分。根据典型周期，CO 的排放速率比 NO 的排放速率高得多，每个周期的排放量分别为 67.7 和 16.5mg。游离氨（FA）对亚硝酸氧化菌的抑制活性和亚硝酸盐积累的发生有重要影响。
