Osborne M P, Comis S D, Johnson A P, Jeffries D R
Department of Physiology, Medical School, University of Birmingham, England.
Acta Otolaryngol. 1989 Sep-Oct;108(3-4):217-26. doi: 10.3109/00016488909125521.
High-resolution scanning electron microscopic studies have been made on the guinea pig cochlea on material fixed from 15 min to 4 h post-mortem. Changes in the surface texture and cross-links of stereocilia were detected after only 15 min. Such changes included an increase in granularity of the surface membrane, thickening, stretching and fracturing of all types of cross-links accompanied by splaying apart and loss of rigidity of stereocilia. These changes were more pronounced in the basal turns of the cochlea and in general increased in severity and spread more apically with increasing times post-mortem. By 4 h, many hair bundles consisted of a fused amorphous mass in which individual stereocilia were not discernible. Remarkably at this time, some hair bundles appeared to have suffered little damage. These results will facilitate better discrimination between effects solely due to post-mortem necrotic changes and those due to specific actions of ototoxic drugs and other insults.
对豚鼠耳蜗进行了高分辨率扫描电子显微镜研究，所用材料为死后15分钟至4小时固定的标本。仅在15分钟后就检测到了静纤毛表面纹理和交联的变化。这些变化包括表面膜颗粒度增加、所有类型交联的增厚、拉伸和断裂，同时伴有静纤毛的张开和刚性丧失。这些变化在耳蜗基底转更为明显，总体上随着死后时间的增加，严重程度增加且向顶侧蔓延更广。到4小时时，许多毛细胞束由融合的无定形团块组成，其中单个静纤毛无法辨认。值得注意的是，此时一些毛细胞束似乎几乎没有受损。这些结果将有助于更好地区分仅由死后坏死变化引起的效应与由耳毒性药物和其他损伤的特定作用引起的效应。
