Fan H J, Duan T H, Li M H
Yao Xue Xue Bao. 1989;24(3):175-81.
Seventeen derivatives of 7-(4,7-di-substituted coumarin-3-acetamido)cephalosporins were synthesized with acid chloride method, Vilsmeier reagent method and mixed anhydride method. Isolation and purification were fulfilled with Sephadex LH-20 column chromatography and centrifugal-TLC technique. Their chemical structures were identified by elemental analysis, IR and 1HNMR spectrum. The preliminary antibacterial activity tests showed that these cephalosporin derivatives exhibited good activity against Gram-positive bacteria and individual Gram-negative bacteria. Further experiments in pharmacology will be made.
采用酰氯法、Vilsmeier试剂法和混合酸酐法合成了17种7-(4,7-二取代香豆素-3-乙酰氨基)头孢菌素衍生物。通过Sephadex LH-20柱色谱和离心薄层色谱技术进行分离纯化。通过元素分析、红外光谱和核磁共振氢谱对其化学结构进行鉴定。初步抗菌活性测试表明，这些头孢菌素衍生物对革兰氏阳性菌和个别革兰氏阴性菌具有良好的活性。将进行进一步的药理学实验。
