Liu L S, Wang P, Wang S Z, Zheng R L, Zhang L D, Chen S Y, Wang C H, Hou Z J
Zhongguo Yao Li Xue Bao. 1989 Mar;10(2):188-91.
The incorporations of [3H] thymidine, [3H]uridine and [3H]leucine into DNA, RNA and protein synthesis in leukemia 7712 cells were inhibited by the complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium (Pr, rare earth element) dicitrite 34 micrograms/ml for 3-24 h. The degree of inhibition increased in proportion to the incubation time. After being treated with [C17H20N2I]3[Pr(C6H5O7)2] 34 micrograms/ml for 3, 6, 12 and 24 h, the incorporation of [32P]Na2HPO4 into the nucleoprotein of leukemia 7712 cells was inhibited by 49, 57, 65 and 85%, while those into ATP were inhibited by 43, 59, 65 and 83%, respectively. The ID50 of [C17H20N2I]3[Pr(C6H5O7)2] on DNA synthesis in leukemia 7712 cells at 24 h was 22 micrograms/ml. After the complex was removed from the medium entirely, the rate of DNA synthesis decreased with time over 3-12 h. This result indicated that the inhibition mechanism was likely due to damage to the DNA template.
3,6-二（二甲基氨基）二苯并碘鎓与柠檬酸镨（Pr，稀土元素）形成的配合物，浓度为34微克/毫升，作用3至24小时，可抑制白血病7712细胞中[3H]胸苷、[3H]尿苷和[3H]亮氨酸掺入DNA、RNA和蛋白质合成。抑制程度随孵育时间成比例增加。用34微克/毫升的[C17H20N2I]3[Pr(C6H5O7)2]处理3、6、12和24小时后，白血病7712细胞核蛋白中[32P]Na2HPO4的掺入分别被抑制49%、57%、65%和85%，而掺入ATP的分别被抑制43%、59%、65%和83%。[C17H20N2I]3[Pr(C6H5O7)2]在24小时对白血病7712细胞DNA合成的半数抑制浓度（ID50）为22微克/毫升。将该配合物从培养基中完全去除后，在3至12小时内DNA合成速率随时间下降。该结果表明抑制机制可能是由于对DNA模板的损伤。
