Li Tingyang, Chu Yang, Yan Kaijing, Li Shuming, Wang Xiangyang, Wang Ying, Li Wei, Ma Xiaohui, Yang Jin, Liu Changxiao
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.
State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.
Biomed Chromatogr. 2017 Jun;31(6). doi: 10.1002/bmc.3889. Epub 2017 Feb 5.
Dantonic pill, consisting of Salviae miltiorrhize, Panax notoginseng and Borneol, is a widely used compound Chinese medicine for preventing and treating ischemic cardiovascular diseases in China. In the present study, an original and sensitive method for simultaneous determination of tanshinol (i.e. danshensu), protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by liquid chromatography-tandem mass spectrometry operated in positive/negative ion switching mode was established and validated. The lower limits of quantification for tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 were 5, 0.5, 1, 0.5, 0.5 and 2 ng/mL, respectively. All of the calibration curves showed good linearity over the investigated concentration range (r > 0.99). Validation results demonstrated that the above compounds were accurately, precisely and robustly quantified in rat plasma. The method was successfully applied to characterize the pharmacokinetic profiles of all six compounds in rats following a single oral administration of Dantonic pill.
丹蒌片由丹参、三七和冰片组成，是中国广泛用于防治缺血性心血管疾病的复方中药。在本研究中，建立并验证了一种采用正/负离子切换模式的液相色谱-串联质谱法同时测定大鼠血浆中丹参素、原儿茶醛、原儿茶酸、三七皂苷R1、人参皂苷Rg1和Rb1的原创性灵敏方法。丹参素、原儿茶醛、原儿茶酸、三七皂苷R1、人参皂苷Rg1和Rb1的定量下限分别为5、0.5、1、0.5、0.5和2 ng/mL。所有校准曲线在研究的浓度范围内均显示出良好的线性（r＞0.99）。验证结果表明，上述化合物在大鼠血浆中能够被准确、精密且稳健地定量。该方法成功应用于单次口服丹蒌片后大鼠体内6种化合物的药代动力学特征研究。
