Bio Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, 350-8585 Japan.
Nanomaterials and Devices Laboratory, Department of Physics, University of Houston, Houston, Texas 77204, USA.
Sci Rep. 2017 Feb 6;7:41844. doi: 10.1038/srep41844.
Nanoarchitectures of titania (TiO) have been widely investigated for a number of medical applications including implants and drug delivery. Although titania is extensively used in the food, drug and cosmetic industries, biocompatibility of nanoscale titania is still under careful scrutiny due to the conflicting reports on its interaction with cellular matter. For an accurate insight, we performed in vitro studies on the response of human dermal fibroblast cells toward pristine titania nanotubes fabricated by anodic oxidation. The nanotubes at low concentrations were seen to induce toxicity to the cells, whereas at higher concentrations the cell vitality remained on par with controls. Further investigations revealed an increase in the G phase cell population depicting that majority of cells were in the resting rather than active phase. Though the mitochondrial set-up did not exhibit any signs of stress, significantly enhanced reactive oxygen species production in the nuclear compartment was noted. The TiO nanotubes were believed to have gained access to the nuclear machinery and caused increased stress leading to genotoxicity. This interesting property of the nanotubes could be utilized to kill cancer cells, especially if the nanotubes are functionalized for a specific target, thus eliminating the need for any chemotherapeutic agents.
TiO 的纳米结构在许多医学应用中得到了广泛的研究，包括植入物和药物输送。尽管 TiO 在食品、药品和化妆品行业得到了广泛的应用，但由于对其与细胞物质相互作用的报告相互矛盾，纳米级 TiO 的生物相容性仍在受到仔细审查。为了更准确地了解情况，我们对人体成纤维细胞对阳极氧化制备的原始 TiO 纳米管的反应进行了体外研究。在低浓度下，纳米管被发现对细胞有毒性，而在较高浓度下，细胞活力与对照组相当。进一步的研究表明，G 期细胞群体增加，表明大多数细胞处于静止而不是活跃状态。尽管线粒体结构没有表现出任何应激迹象，但核区活性氧物质的产生明显增加。据认为，TiO 纳米管已经进入核机制并导致增加的应激导致遗传毒性。纳米管的这一有趣特性可用于杀死癌细胞，特别是如果纳米管经过特定靶标功能化，从而无需任何化疗药物。
