Takeda Kazuki, Miki Kunio
Department of Chemistry, Graduate School of Science, Kyoto University, Japan.
FEBS J. 2017 Jul;284(14):2163-2166. doi: 10.1111/febs.14036. Epub 2017 Feb 17.
The high-potential iron-sulfur protein (HiPIP) is a small (~ 80 residues) soluble metalloprotein functioning as an electron carrier in photosynthetic bacteria. HiPIP has one Fe S cluster at its molecular center. Its electronic structure is important for understanding electron transport. We recently succeeded in determining an ultra-high-resolution structure and analyzing the charge-density of HiPIP by using X-ray diffraction data at 0.48 Å resolution. The distribution of valence electrons in the iron-sulfur cluster and in the protein environment were clearly visualized, which is the first successful case for metalloproteins. In addition, a topological analysis of the charge density provided information about the electronic structure of the cluster.
高电位铁硫蛋白（HiPIP）是一种小型（约80个残基）可溶性金属蛋白，在光合细菌中作为电子载体发挥作用。HiPIP在其分子中心有一个铁硫簇。其电子结构对于理解电子传输很重要。我们最近通过使用分辨率为0.48 Å的X射线衍射数据成功确定了HiPIP的超高分辨率结构并分析了其电荷密度。铁硫簇和蛋白质环境中的价电子分布清晰可见，这是金属蛋白的首个成功案例。此外，电荷密度的拓扑分析提供了有关该簇电子结构的信息。
