Vilariño M, Cuadro F, Dos Santos-Neto P C, García-Pintos C, Menchaca A
Instituto de Reproducción Animal Uruguay - Fundación IRAUy, Montevideo, Uruguay.
Instituto de Reproducción Animal Uruguay - Fundación IRAUy, Montevideo, Uruguay.
Theriogenology. 2017 Mar 1;90:163-168. doi: 10.1016/j.theriogenology.2016.12.003. Epub 2016 Dec 3.
The objective of the present study was to determine the ovarian response induced with the prostaglandin-based protocol Synchrovine (two doses of PGF given 7 d apart), as well as the fertility after FTAI. In Experiment 1, 15 females received the Synchrovine protocol using two different PGF analogues (Delprostenate vs. D-Cloprostenol). No differences in estrus response, time of ovulation and follicular dynamics were found between both groups (P < 0.05). The ovulation after Synchrovine was synchronized in a similar mean interval (68.8 ± 7.1 h) than when the females received a single dose of PGF (70.2 ± 20.7 h; P=NS), but the dispersion between the first and the last ovulation was reduced with this protocol (range 60-84 h vs. 24-96 h, respectively; P < 0.05). In experiment 2, 318 ewes were treated with the Synchrovine protocol and cervical FTAI was performed using different sperm cell concentrations. Pregnancy rate was higher using 200 × 10 and 100 × 10 sperm cells (38.2%, 39/102; and 34.9%, 38/109, respectively) than using 50 × 10 (23.4%, 25/107, P < 0.05). In Experiment 3, 444 ewes received the Synchrovine protocol and were assigned to receive 300 IU of eCG or not at the moment of the second dose of PGF, and cervical FTAI was performed 42 h or 48 h after the second dose of PGF. No effect was found related to the eCG administration nor the time of insemination. In Experiment 4, 342 received cervical or intrauterine insemination after treatment with the Synchrovine protocol, resulting in greater pregnancy rate for intrauterine insemination than cervical insemination (52.5%, 90/171 vs. 31%, 53/171, P < 0.05). These experiments demonstrate that the Synchrovine protocol effectively induces luteolysis, estrus and ovulation in most of the treated females, and ovulation is synchronized into a narrow window of 24 h. Pregnancy rate obtained with cervical FTAI is around 30-45%, with similar results using 100 × 10 or 200 × 10 sperm cells, the eCG administration seems not to be necessary, the type of PGF2α analogue does not appear relevant, and fertility is improved with intrauterine semen deposition.
本研究的目的是确定基于前列腺素的同步发情方案（间隔7天注射两剂前列腺素F2α）诱导的卵巢反应，以及定时人工授精后的繁殖力。在实验1中，15只雌性动物使用两种不同的前列腺素F2α类似物（氯前列醇钠与氯前列烯醇）接受同步发情方案。两组在发情反应、排卵时间和卵泡动态方面均未发现差异（P<0.05）。同步发情方案后的排卵同步化平均间隔（68.8±7.1小时）与雌性动物接受单剂量前列腺素F2α时相似（70.2±20.7小时；P=无显著差异），但该方案使第一次和最后一次排卵之间的离散度降低（范围分别为60 - 84小时和24 - 96小时；P<0.05）。在实验2中，318只母羊接受同步发情方案，并使用不同精子细胞浓度进行子宫颈定时人工授精。使用200×10⁶和100×10⁶精子细胞时的妊娠率较高（分别为38.2%，39/102；和34.9%，38/109），高于使用50×10⁶精子细胞时的妊娠率（23.4%，25/107，P<0.05）。在实验3中，444只母羊接受同步发情方案，并在第二次注射前列腺素F2α时被分配接受或不接受300国际单位的孕马血清促性腺激素，在第二次注射前列腺素F2α后42小时或48小时进行子宫颈定时人工授精。未发现与孕马血清促性腺激素给药及授精时间相关的影响。在实验4中，342只母羊在接受同步发情方案治疗后接受子宫颈或子宫内授精，结果显示子宫内授精的妊娠率高于子宫颈授精（52.5%，90/171对31%，53/171，P<0.05）。这些实验表明，同步发情方案能有效诱导大多数接受治疗的雌性动物黄体溶解、发情和排卵，且排卵同步到24小时的狭窄窗口内。子宫颈定时人工授精获得的妊娠率约为30% - 45%，使用100×10⁶或200×10⁶精子细胞时结果相似，孕马血清促性腺激素给药似乎没有必要，前列腺素F2α类似物的类型似乎无关紧要，子宫内精液沉积可提高繁殖力。
