Pascuan C G, Di Rosso M E, Pivoz-Avedikian J E, Wald M R, Zorrilla Zubilete M A, Genaro A M
Instituto de Genética "Ewald A. Favret" (IGEAF), Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVYA), Nicolas Repetto y de los Reseros s/n, 1686 Hurlingham, Provincia de Buenos Aires, Argentina.
Instituto de Investigaciones Biomédicas (BIOMED), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Católica Argentina (UCA), Av. Alicia Moreau de Justo 1600, Piso 3, 1107 Buenos Aires, Argentina.
Physiol Behav. 2017 May 1;173:144-155. doi: 10.1016/j.physbeh.2017.01.045. Epub 2017 Feb 4.
Much evidence has suggested that early life adversity can have a lasting effect on behavior. The aim of this study was to explore the impact of prenatal exposure to stress on cognition in adult life and how it impacts chronic stress situations. In addition, we investigated the participation of glucocorticoids, neurotrophins and cytokines in prenatal stress effects. For this purpose, pregnant mice were placed in a cylindrical restraint tube for 2h daily during the last week of pregnancy. Control pregnant females were left undisturbed during their entire pregnancy period. Object-in-place task results showed that adult female mice exposed to prenatal stress exhibited an impairment in spatial memory. However, in the alternation test this memory deficit was only found in prenatally stressed mice submitted to chronic stress. This alteration occurred in parallel with a decrease in BDNF, an increase in glucocorticoid receptors and an alteration of Th1/Th2 in the hippocampus. Interestingly, these changes were observed in peripheral lymph nodes as well. However, none of the mentioned changes were observed in adult male mice. These results indicate that lymphoid cells could be good candidates as peripheral markers of susceptibility to behavioral alterations associated with prenatal exposure to stress.
许多证据表明，早期生活逆境会对行为产生持久影响。本研究的目的是探讨孕期暴露于应激对成年后认知的影响以及它如何影响慢性应激情况。此外，我们研究了糖皮质激素、神经营养因子和细胞因子在孕期应激效应中的作用。为此，在怀孕的最后一周，将怀孕小鼠每天置于圆柱形束缚管中2小时。对照怀孕雌性小鼠在整个孕期不受干扰。位置记忆任务结果表明，暴露于孕期应激的成年雌性小鼠表现出空间记忆受损。然而，在交替试验中，这种记忆缺陷仅在遭受慢性应激的孕期应激小鼠中发现。这种改变与海马中脑源性神经营养因子（BDNF）的减少、糖皮质激素受体的增加以及Th1/Th2的改变同时发生。有趣的是，在周围淋巴结中也观察到了这些变化。然而，在成年雄性小鼠中未观察到上述任何变化。这些结果表明，淋巴细胞可能是与孕期暴露于应激相关的行为改变易感性的良好外周标志物候选者。
