Wu Tsung-Ru, Huang Tsung-Teng, Martel Jan, Liau Jian-Ching, Chiu Chen-Yaw, Leu Yann-Lii, Jian Wei-Ting, Chang I-Te, Lu Chia-Chen, Ojcius David M, Ko Yun-Fei, Lai Hsin-Chih, Young John D
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 33302, Taiwan.
J Ethnopharmacol. 2017 Apr 6;201:117-122. doi: 10.1016/j.jep.2017.02.008. Epub 2017 Feb 4.
The medicinal mushroom Antrodia cinnamomea possesses anticancer properties but the active compounds responsible for these effects are mostly unknown.
We aimed to identify novel A. cinnamomea compounds that produce cytotoxic effects on cancer cells.
Using ethanol extraction and chromatography, we isolated the lanostanoid compound lanosta-7,9(11),24-trien-3β,15α,21-triol (1) from cultured A. cinnamomea mycelium. Cytotoxicity and pro-apoptotic effects of compound 1 were evaluated using the MTS assay and flow cytometry analysis, respectively.
Compound 1 produced cytotoxic effects on the nasopharyngeal carcinoma cell lines TW02 and TW04, with IC values of 63.3 and 115.0μM, respectively. On the other hand, no cytotoxic effects were observed on non-tumorigenic nasopharyngeal epithelial cells (NP69). In addition, compound 1 induced apoptosis in TW02 and TW04 cells as revealed by flow cytometry analysis.
Our results demonstrate for the first time the presence of pinicolol B in A. cinnamomea mycelium and suggest that this compound may contribute to the anticancer effects of A. cinnamomea.
药用真菌樟芝具有抗癌特性，但导致这些作用的活性化合物大多未知。
我们旨在鉴定对癌细胞产生细胞毒性作用的新型樟芝化合物。
通过乙醇提取和色谱法，我们从培养的樟芝菌丝体中分离出羊毛甾烷型化合物羊毛甾-7,9(11),24-三烯-3β,15α,21-三醇（1）。分别使用MTS法和流式细胞术分析评估化合物1的细胞毒性和促凋亡作用。
化合物1对鼻咽癌细胞系TW02和TW04产生细胞毒性作用，IC值分别为63.3和115.0μM。另一方面，在非致瘤性鼻咽上皮细胞（NP69）中未观察到细胞毒性作用。此外，流式细胞术分析显示化合物1诱导TW02和TW04细胞凋亡。
我们的结果首次证明樟芝菌丝体中存在松苓新酸B，并表明该化合物可能有助于樟芝的抗癌作用。
