高密度脂蛋白水平正常化后，循环中富含心脏的微小RNA的诊断敏感性增加。

The diagnostic sensitivity of circulating cardio-enriched microRNAs is increased after normalization of high-density lipoprotein levels.

作者信息

Ram Thrishila Parshu, Fomison-Nurse Ingrid, Gandhi Sophie, Coffey Sean, Saxena Pankaj, Galvin Ivor, Bunton Richard, Williams Michael J A, Lamberts Regis R, Katare Rajesh

机构信息

Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

出版信息

Int J Cardiol. 2017 Jun 1;236:498-500. doi: 10.1016/j.ijcard.2017.01.119. Epub 2017 Jan 26.

DOI:10.1016/j.ijcard.2017.01.119

PMID:28169059

Abstract

BACKGROUND

Emerging evidence suggests that microRNAs (miRs) could be a potential biomarker to identify early molecular alterations in the heart. HDL are the major carriers for miRs into the circulation. This study tested whether changes in the level of HDL could affect the diagnostic sensitivity of miRs.

METHODS AND RESULTS

Peripheral blood samples were collected from 20 diabetic and 22 age and gender matched non-diabetic patients undergoing coronary artery bypass graft (CABG) surgery for ischemic heart disease (IHD). Total RNA was extracted from the separated plasma and stored in -80°C. Reverse transcription and amplification using specific primers against cardio-enriched miR-1, -34a, -126, -133, and -499 showed significant correlation between HDL levels and miR-1, -133 and -499. Importantly, normalization of miR levels with HDL showed a significant downregulation of miR-1, -133 and -499 in diabetic plasma, which was not observed before normalization with HDL levels.

CONCLUSION

Normalization of circulating miR levels with HDL increases the diagnostic sensitivity of circulating miRs.

摘要

背景

新出现的证据表明，微小RNA（miRs）可能是识别心脏早期分子改变的潜在生物标志物。高密度脂蛋白（HDL）是miRs进入循环系统的主要载体。本研究测试了HDL水平的变化是否会影响miRs的诊断敏感性。

方法与结果

从20例因缺血性心脏病（IHD）接受冠状动脉旁路移植术（CABG）的糖尿病患者和22例年龄及性别匹配的非糖尿病患者中采集外周血样本。从分离的血浆中提取总RNA并储存在-80°C。使用针对心脏富集的miR-1、-34a、-126、-133和-499的特异性引物进行逆转录和扩增，结果显示HDL水平与miR-1、-133和-499之间存在显著相关性。重要的是，用HDL对miR水平进行标准化后，糖尿病血浆中miR-1、-133和-499显著下调，而在未用HDL水平进行标准化之前未观察到这种情况。