Bozdogan Elif, Topcuoglu Nursen, Cetin Gurkan, Haberal Ismail, Kulekci Guven, Aktoren Oya
Department of Pediatric Dentistry, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
Department of Microbiology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
Indian J Dent Res. 2016 Nov-Dec;27(6):637-642. doi: 10.4103/0970-9290.199590.
The purpose of this study was to analyze the presence of Aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children requiring cardiac surgery in Istanbul, Turkey.
Twenty-five patients (mean age: 6.24 ± 2.93) undergoing surgery for congenital heart defects (CHDs) and an age/gender-matched control group of 25 healthy children were enrolled in the study. Saliva samples were collected from all children; plaque index (PI) and gingival index (GI) were also determined. In CHD group, cardiac tissue samples were received during surgery. All samples were evaluated for the presence of A. actinomycetemcomitans and its highly leukotoxic JP2 clonal strains using polymerase chain reaction. The findings were analyzed by Mann-Whitney U, Chi-square, and Fisher's exact tests.
No significant differences were found in PI and GI values between the groups. A. actinomycetemcomitans was not detected in cardiac tissue samples. A. actinomycetemcomitans in saliva was detected in 2 (8%) of the CHD and 5 (20%) of the control children (p > 0.05). A. actinomycetemcomitans JP2 clonal strains were determined from 1 (4%) of the control group while it was not determined from the samples of the CHD group.
Early colonization of A. actinomycetemcomitans in oral cavities could be assessed as a risk marker for periodontal disease. Periodontal pathogens may enter bloodstream through bacteremia; thus, the presence of periodontal pathogens in the oral cavity of children should be assessed as a risk marker for cardiac diseases in older ages.
本研究旨在分析土耳其伊斯坦布尔需要进行心脏手术的儿童唾液和心脏组织样本中伴放线聚集杆菌的存在情况。
本研究纳入了25例接受先天性心脏病（CHD）手术的患者（平均年龄：6.24±2.93岁）以及25名年龄/性别匹配的健康儿童作为对照组。采集了所有儿童的唾液样本；还测定了菌斑指数（PI）和牙龈指数（GI）。在CHD组中，手术期间采集了心脏组织样本。使用聚合酶链反应评估所有样本中伴放线聚集杆菌及其高白细胞毒性JP2克隆菌株的存在情况。通过Mann-Whitney U检验、卡方检验和Fisher精确检验分析研究结果。
两组之间的PI和GI值没有显著差异。在心脏组织样本中未检测到伴放线聚集杆菌。在CHD组的2名（8%）儿童和对照组的5名（20%）儿童的唾液中检测到伴放线聚集杆菌（p>0.05）。从1名（4%）对照组儿童中检测到伴放线聚集杆菌JP2克隆菌株，而在CHD组样本中未检测到。
伴放线聚集杆菌在口腔中的早期定植可被评估为牙周疾病的风险标志物。牙周病原体可能通过菌血症进入血液；因此，儿童口腔中牙周病原体的存在应被评估为老年心脏病的风险标志物。
