L细胞中钾转运的基因改变。
Genetic alterations in potassium transport in L cells.
作者信息
Gargus J J, Miller I L, Slayman C W, Adelberg E A
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5589-93. doi: 10.1073/pnas.75.11.5589.
Abstract
Starting with mutagenized cultures of the mouse fibroblastic cell line LM(TK-), we have selected mutant clones by their ability to grow at 0.2 micrometer K+, a concentration unable to support the growth of the parent cell. The mutants fall into two classes on the basis of their potassium transport properties. Both classes maintain a high intracellular K+ concentration when growing in low-potassium medium, and both are unaltered in the ouabain-sensitive Na/K pump. One class shows an increased activity of a ouabain-resistant, furosemide-sensitive K+ transport system; the other class shows a decreased activity of a specific component of K+ efflux.
摘要
从诱变的小鼠成纤维细胞系LM(TK-)培养物开始，我们通过它们在0.2微摩尔钾离子浓度下生长的能力筛选出突变克隆，该浓度无法支持亲本细胞的生长。根据它们的钾转运特性，这些突变体分为两类。两类突变体在低钾培养基中生长时都能维持较高的细胞内钾离子浓度，并且两者在哇巴因敏感的钠钾泵方面均未改变。一类显示出对哇巴因耐药、对呋塞米敏感的钾离子转运系统活性增加；另一类显示出钾离子外流特定成分的活性降低。
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