对正常男性受试者的红细胞进行了两次研究，测定了细胞内钠、钾和镁的浓度、哇巴因敏感的86铷摄取、钠外流以及钠钾协同转运活性。

Intracellular sodium, potassium and magnesium concentration, ouabain-sensitive 86rubidium-uptake and sodium-efflux and Na+, K+-cotransport activity in erythrocytes of normal male subjects studied on two occasions.

作者信息

Lijnen P, Hespel P, Lommelen G, Laermans M, M'Buyamba-Kabangu J R, Amery A

出版信息

Methods Find Exp Clin Pharmacol. 1986 Sep;8(9):525-33.

Abstract

The red cell Na+,K+-ATPase pump activity estimated by the ouabain-sensitive 86Rb-uptake or Na+-efflux, the Na+,K+-cotransport activity measured either by the furosemide-sensitive K+- or Na+- efflux or by the ethacrynic acid-sensitive 86Rb-uptake as well as the intraerythrocyte concentration of sodium, potassium and magnesium were studied in 29 normal male subjects with one to three weeks interval between the first and second blood sampling. Both the red cell sodium and potassium concentration, the erythrocyte ouabain-sensitive 86Rb-uptake and Na+-efflux, the furosemide-sensitive Na+- and K+-efflux and the ethacrynic acid-sensitive 86Rb-uptake are stable over time in the same individual. The furosemide-sensitive Na+-and K+-efflux is significantly related to the ethacrynic acid-sensitive 86Rb-uptake. The intraerythrocyte Na+ concentration was negatively related to the ouabain-sensitive 86Rb-uptake, but not to the ouabain-sensitive Na+-efflux; it was, however, negatively related to the rate constant for the ouabain-sensitive Na+-efflux.

摘要

通过哇巴因敏感的⁸⁶Rb摄取或Na⁺外流来估计红细胞Na⁺,K⁺-ATP酶泵活性，通过呋塞米敏感的K⁺或Na⁺外流或依他尼酸敏感的⁸⁶Rb摄取来测量Na⁺,K⁺协同转运活性，以及研究红细胞内钠、钾和镁的浓度，这些研究针对29名正常男性受试者，在第一次和第二次采血之间间隔一到三周。红细胞钠和钾浓度、红细胞哇巴因敏感的⁸⁶Rb摄取和Na⁺外流、呋塞米敏感的Na⁺和K⁺外流以及依他尼酸敏感的⁸⁶Rb摄取在同一个体中随时间推移是稳定的。呋塞米敏感的Na⁺和K⁺外流与依他尼酸敏感的⁸⁶Rb摄取显著相关。红细胞内Na⁺浓度与哇巴因敏感的⁸⁶Rb摄取呈负相关，但与哇巴因敏感的Na⁺外流无关；然而，它与哇巴因敏感的Na⁺外流的速率常数呈负相关。