White P
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Am J Otolaryngol. 1989 Sep-Oct;10(5):301-4. doi: 10.1016/0196-0709(89)90103-8.
Three solutions, surfactant prepared from pig lungs, a mixture of synthetic phospholipids, and saline solution, were instilled into the middle ears of rats. The middle ear pressure necessary to force the eustachian tube open was studied. Ten minutes after instillation of pulmonary surfactant there was a 0.58 +/- 0.10 kPa decrease in the pressure opening level (P less than .001). The reduction persisted throughout the 50-minute experiment. There was also a reduction 0.17 +/- 0.16 kPa in the pressure opening level (P less than .01) after the administration of the synthetic phospholipid mixture. This reduction was significantly less than the reduction produced by the pulmonary surfactant. No decrease in the pressure opening level was seen after the instillation of saline solution into the middle ear cavity. The fact that surfactants are demonstrable in the middle ear and eustachian tube, coupled with these findings of an effect on the tubal opening, suggests a physiologic role for surface tension-lowering substances in tubal function.
将三种溶液，即从猪肺制备的表面活性剂、合成磷脂混合物和盐溶液，滴入大鼠中耳。研究了迫使咽鼓管打开所需的中耳压力。滴入肺表面活性剂10分钟后，压力打开水平下降了0.58±0.10千帕（P＜0.001）。在整个50分钟的实验过程中，这种下降持续存在。给予合成磷脂混合物后，压力打开水平也下降了0.17±0.16千帕（P＜0.01）。这种下降明显小于肺表面活性剂产生的下降。向中耳腔滴入盐溶液后，未观察到压力打开水平下降。中耳和咽鼓管中可检测到表面活性剂，再加上这些对咽鼓管开放有影响的发现，表明降低表面张力的物质在咽鼓管功能中具有生理作用。
