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Determination of intracellular pyridine nucleotide levels by bioluminescence using anaerobic bacteria as a model.

作者信息

Schmid U, Schimz K L, Sahm H

机构信息

Institut für Biotechnologie 1 der KFA Jülich GmbH, Federal Republic of Germany.

出版信息

Anal Biochem. 1989 Jul;180(1):17-23. doi: 10.1016/0003-2697(89)90082-1.

DOI:10.1016/0003-2697(89)90082-1
PMID:2817339
Abstract

An assay for the determination of NAD(P)+ and NAD(P)H in extracts from the obligate anaerobe bacterium Thermoanaerobacter finnii is developed and the strategy for this development is described. This assay performed with constant FMN reductase (EC 1.6.8.1) and luciferase (EC 1.14.14.3) concentrations has been shown to detect as low as 1 pmol pyridine nucleotide. With this assay recovery, efficiency of extraction, reliability, and detection limit of the procedures were determined. To our knowledge this is the first bioluminometric assay for the determination of pyridine nucleotide levels in bacterial extracts.

摘要

相似文献

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Determination of intracellular pyridine nucleotide levels by bioluminescence using anaerobic bacteria as a model.
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2
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[Bioluminescent method of determining picomolar amounts of nicotinamide-adenine dinucleotide using an immobilized extract of the luminescent bacterium Beneckea harveyi].[使用哈维贝内克氏菌发光细菌的固定化提取物测定皮摩尔量烟酰胺腺嘌呤二核苷酸的生物发光方法]
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Affinity purification of bacterial luciferase and NAD(P)H:FMN oxidoreductases by FMN-sepharose for analytical applications.通过FMN-琼脂糖亲和纯化细菌荧光素酶和NAD(P)H:FMN氧化还原酶用于分析应用。
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Anal Biochem. 1992 Apr;202(1):162-5. doi: 10.1016/0003-2697(92)90222-s.

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