如何构建一台用于自动化聚合酶链反应的廉价循环温控仪器。

How to build an inexpensive cyclotherm instrument for automated polymerase chain reaction.

作者信息

Collasius M, Falk H, Ciesler C, Valet G

机构信息

Mildred-Scheel-Labor für Krebszellforschung, Max-Planck-Institut, Martinsried, Federal Republic of Germany.

出版信息

Anal Biochem. 1989 Aug 15;181(1):163-6. doi: 10.1016/0003-2697(89)90411-9.

DOI:10.1016/0003-2697(89)90411-9

PMID:2817375

Abstract

The Cyclotherm instrument is a functionally fully equivalent but inexpensive alternative to commercial instruments for automated polymerase chain reaction (PCR). It can be rebuilt under conditions of a biochemical laboratory for less than +1000. A Peltier element is used for heating and cooling of the reaction vials and the temperature and timing of the PCR cycles are controlled by a BASIC program in a SHARP PC 1600 low cost computer.

摘要

循环温度仪是一种功能上与商业自动化聚合酶链反应（PCR）仪器完全等效但价格低廉的替代品。它可以在生化实验室条件下以不到1000美元的成本进行组装。珀尔帖元件用于加热和冷却反应管，PCR循环的温度和时间由夏普PC 1600低成本计算机中的BASIC程序控制。