Rasmussen Karina Juhl, Skjoedt Mikkel-Ole, Vitved Lars, Skjoedt Karsten, Palarasah Yaseelan
Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
Department of Clinical Immunology, University Hospital of Copenhagen, Rigshospitalet, Denmark.
J Immunol Methods. 2017 May;444:51-55. doi: 10.1016/j.jim.2017.02.002. Epub 2017 Feb 4.
The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysiological mechanisms. We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3. We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics.
补体成分C3及其裂解产物C3b/iC3b、C3c和C3d在临床诊断中用作生物标志物。目前，尚无特异性抗体能够区分C3d与其他片段，尽管考虑到它们可能反映不同的病理生理机制，这种区分可能非常有价值。我们已经开发出一种大鼠抗人C3d单克隆抗体，它对C3d N端区域的末端序列具有特异性。因此，该抗体只有在C3裂解的最终终产物形式为C3d时才能与之结合。我们认为这种特异性是同类中的首例，并预计它可作为一种检测工具用于多种免疫方法，在诊断中具有重要价值。
