Veiga Andreza, Albuquerque Kelly, Corrêa Maria Elinete, Brigido Helliton, Silva E Silva João, Campos Marliane, Silveira Fernando, Santos Lourivaldo, Dolabela Maria
Programa de Pós-Graduação em Inovação Farmacêutica, Instituto de Ciências da Saúde, Universidade Federal do Pará, Rua Augusto Corrêa, 01, 66075-110, Belém, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Rua Augusto Corrêa, 01, 66075-110, Belém, Brazil.
Exp Parasitol. 2017 Apr;175:68-73. doi: 10.1016/j.exppara.2017.02.005. Epub 2017 Feb 4.
Virola species have been used in traditional medicine as healing in skin infections. From V. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of Leishmania. The current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of Virola surinamensis. Hexane, Ethyl Acetate, and Methanol extracts were obtained from powder of dry leaves of V. surinamensis. The hexane and ethyl acetate extracts were fractionated by silica gel column chromatography and increasingly polar gradient. The viability of L. chagasi and L. amazonensis promastigotes was assessed by tetrazolium salt assay (MTT). Peritoneal macrophages were exposed to L. amazonensis promastigotes. The treatment was performed with the extracts for 24 h. Then, the coverslips were stained and the infection index was determined. Cytotoxicity was determined in macrophage cells by peritoneum viability assay (MTT). The selectivity index was calculated as the product of cytotoxic concentration 50% and inhibitory concentration 50%. The hexane extract showed leishmanicide activity in promastigotes. The ethyl acetate, methanol extracts and fractions (C1-C6), were inactive against promastigote form of L. chagasi and L. amzazonensis. None extract showed effect on L. amazonensis amastigotes. All samples tested showed low cytotoxicity (CC > 500 μg/mL). The selectivity index of the hexane extract was greater than 5. The hexane extract of V. surinamensis was active against L. chagasi and L. amazonensis promastigotes. The extract fractionation did not increase significantly its antipromastigote activity. The surinamensin is probably not responsible for the activity. The extracts were inactive against amastigotes of L. amazonensis.
维罗勒属植物在传统医学中被用于治疗皮肤感染。从苏里南维罗勒油中分离出了几种倍半萜，如 Nerolidol，其对利什曼原虫属物种具有活性。本研究旨在评估从苏里南维罗勒叶片中获得的提取物、馏分和苏里南菌素的杀利什曼原虫活性及毒性。从苏里南维罗勒干叶粉末中获得了己烷、乙酸乙酯和甲醇提取物。己烷和乙酸乙酯提取物通过硅胶柱色谱和逐渐增加极性的梯度进行分离。通过四唑盐测定法（MTT）评估恰加斯利什曼原虫和亚马逊利什曼原虫前鞭毛体的活力。将腹腔巨噬细胞暴露于亚马逊利什曼原虫前鞭毛体。用提取物进行处理 24 小时。然后，对盖玻片进行染色并测定感染指数。通过腹膜活力测定法（MTT）在巨噬细胞中测定细胞毒性。选择性指数计算为细胞毒性浓度 50%与抑制浓度 50%的乘积。己烷提取物在前鞭毛体中显示出杀利什曼原虫活性。乙酸乙酯、甲醇提取物和馏分（C1 - C6）对恰加斯利什曼原虫和亚马逊利什曼原虫的前鞭毛体形式无活性。没有提取物对亚马逊利什曼原虫无鞭毛体显示出作用。所有测试样品均显示出低细胞毒性（CC＞500μg/mL）。己烷提取物的选择性指数大于 5。苏里南维罗勒的己烷提取物对恰加斯利什曼原虫和亚马逊利什曼原虫前鞭毛体具有活性。提取物分级分离并未显著增加其抗前鞭毛体活性。苏里南菌素可能与该活性无关。这些提取物对亚马逊利什曼原虫无鞭毛体无活性。
