Edelson J D, Shannon J M, Mason R J
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Am Rev Respir Dis. 1989 Nov;140(5):1398-404. doi: 10.1164/ajrccm/140.5.1398.
Previous observations have suggested that differentiated functions of adult rat type II alveolar cells are affected in part by cell-matrix interactions. We examined several aspects of differentiated adult human type II cells cultured on either bovine corneal endothelial cell extracellular matrix (BCECM) or matrix derived from the Englebreth-Holm-Swarm tumor (EHS). Compared to cells cultured on BCECM, adult human type II cells grown on EHS assumed a more cuboidal shape, had a more defined apical-basal polarity, and appeared to contain a greater number of lamellar bodies and neutral lipid inclusions. These cells also incorporated a greater percentage of [14C]acetate into saturated phosphatidylcholine (SPC) than did their counterparts grown on BCECM. In contrast, the relative incorporation of [14C]acetate into phosphatidylglycerol (PG) was lower in cells grown on EHS than cells cultured on BCECM. The histochemical stain for alkaline phosphatase was useful in identification of human type II cells. Alkaline phosphatase expression was elevated in cells cultured on EHS compared to those cultured on BCECM. These results suggest that maintenance of a differentiated morphology, lipid synthesis, and expression of alkaline phosphatase activity by primary cultures of adult human type II cells are also influenced by cell-matrix interactions. All markers of differentiated function of type II cells except synthesis of PG are better maintained on EHS than on BCECM. Under the conditions of these experiments, synthesis of SPC and PG appears to be independently regulated.
先前的观察表明，成年大鼠II型肺泡细胞的分化功能部分受细胞与基质相互作用的影响。我们研究了在牛角膜内皮细胞细胞外基质（BCECM）或Englebreth-Holm-Swarm肿瘤（EHS）来源的基质上培养的成年人类II型细胞分化的几个方面。与在BCECM上培养的细胞相比，在EHS上生长的成年人类II型细胞呈更立方形的形状，具有更明确的顶-基极性，并且似乎含有更多的板层小体和中性脂质包涵体。这些细胞也比在BCECM上生长的对应细胞将更大比例的[14C]乙酸掺入饱和磷脂酰胆碱（SPC）中。相比之下，在EHS上生长的细胞中[14C]乙酸掺入磷脂酰甘油（PG）的相对比例低于在BCECM上培养的细胞。碱性磷酸酶的组织化学染色有助于鉴定人类II型细胞。与在BCECM上培养的细胞相比，在EHS上培养的细胞中碱性磷酸酶表达升高。这些结果表明，成年人类II型细胞原代培养物中分化形态的维持、脂质合成以及碱性磷酸酶活性的表达也受细胞与基质相互作用的影响。II型细胞分化功能的所有标志物，除了PG的合成外，在EHS上比在BCECM上维持得更好。在这些实验条件下，SPC和PG的合成似乎是独立调节的。
