Hädener Marianne, Weinmann Wolfgang, van Staveren Dave R, König Stefan
Institute of Forensic Medicine, University of Bern, Bühlstrasse 20, 3012 Bern, Switzerland.
Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf, Switzerland.
Bioanalysis. 2017 Mar;9(5):485-496. doi: 10.4155/bio-2016-0281. Epub 2017 Feb 8.
Generally, urine drug testing for cannabis abuse involves measuring total concentrations of 11-nor-9-carboxy-Δ-tetrahydrocannabinol (THCCOOH) obtained by enzymatic and/or alkaline hydrolysis of THCCOOH-glucuronide. As hydrolysis can be inconsistent and incomplete, direct measurement of the two metabolites is preferable. Methodology & results: We developed a high-throughput LC-MS/MS method for simultaneous quantification of free and glucuronidated THCCOOH in urine using coated 96-well plates for analyte extraction and column-switching chromatography. Excellent separation of the two analytes was achieved within 2.5 min, with linear ranges from 5 to 2000 μg/l for THCCOOH and from 10 to 4000 μg/l for THCCOOH-glucuronide.
The method was successfully validated and applied to authentic urine samples from cannabis consumers, demonstrating its applicability for routine cannabinoid testing.
一般来说，针对大麻滥用的尿液药物检测涉及测量通过对四氢大麻酚葡糖苷酸（THCCOOH）进行酶解和/或碱解而获得的11-去甲-9-羧基-Δ-四氢大麻酚（THCCOOH）的总浓度。由于水解可能不一致且不完全，因此直接测量这两种代谢物更为可取。方法与结果：我们开发了一种高通量液相色谱-串联质谱法，用于同时定量尿液中游离和葡糖醛酸化的THCCOOH，使用包被的96孔板进行分析物提取和柱切换色谱法。在2.5分钟内实现了两种分析物的出色分离，THCCOOH的线性范围为5至2000μg/l，THCCOOH-葡糖苷酸的线性范围为10至4000μg/l。
该方法已成功验证并应用于大麻使用者的真实尿液样本，证明了其在常规大麻素检测中的适用性。
