Oliveira de Lima Vanessa Cristina, de Araújo Machado Richele Janaína, Vieira Monteiro Norberto Kássio, de Lyra Ibson Lucas, da Silva Camillo Christina, Coelho Serquiz Alexandre, Silva de Oliveira Adeliana, da Silva Rufino Fabíola Patrícia, Leal Lima Maciel Bruna, Ferreira Uchôa Adriana, Antunes Dos Santos Elizeu, de Araújo Morais Ana Heloneida
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
b Morphology Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Biochem Cell Biol. 2017 Apr;95(2):243-250. doi: 10.1139/bcb-2016-0034. Epub 2016 Sep 12.
Trypsin and chymotrypsin inhibitors from Erythrina velutina seeds have been previously isolated by our group. In previous studies using a sepsis model, we demonstrated the antitumor and anti-inflammatory action of these compounds. This study aimed to evaluate the gastroprotective and antielastase effects of protein inhibitors from E. velutina seeds in an experimental stress-induced ulcer model. Two protein isolates from E. velutina seeds, with antitrypsin (PIAT) and antichymotrypsin (PIAQ) activities, were tested. Both protein isolates showed a high affinity and inhibitory effect against human neutrophil elastase, with 84% and 85% inhibition, respectively. Gastric ulcer was induced using ethanol (99%) in 6 groups of animals (female Wistar rats, n = 6). Before ulcer induction, these animals were treated for 5 days with one of the following: (1) PIAT (0.2 mg·kg), (2) PIAT (0.4 mg·kg), (3) PIAQ (0.035 mg·kg), (4) ranitidine hydrochloride (50 mg·kg), (5) saline solution (0.9%), or (6) no intervention (sham). Both PIAT and PIAQ protected gastric mucosa, preventing hemorrhagic lesions, edema, and mucus loss. No histologic toxic effects of PIAT or PIAQ were seen in liver and pancreatic cells. Our results show that protein isolates from E. velutina seeds have potential gastroprotective effects, placing these compounds as natural candidates for gastric ulcer prevention.
此前，我们团队已从绒毛刺桐种子中分离出胰蛋白酶和胰凝乳蛋白酶抑制剂。在先前使用脓毒症模型的研究中，我们证明了这些化合物的抗肿瘤和抗炎作用。本研究旨在评估绒毛刺桐种子中的蛋白质抑制剂在实验性应激诱导溃疡模型中的胃保护和抗弹性蛋白酶作用。对绒毛刺桐种子中的两种具有抗胰蛋白酶（PIAT）和抗胰凝乳蛋白酶（PIAQ）活性的蛋白质分离物进行了测试。两种蛋白质分离物均对人中性粒细胞弹性蛋白酶表现出高亲和力和抑制作用，抑制率分别为84%和85%。用99%乙醇诱导6组动物（雌性Wistar大鼠，n = 6）产生胃溃疡。在诱导溃疡前，用以下之一对这些动物进行5天的治疗：（1）PIAT（0.2 mg·kg），（2）PIAT（0.4 mg·kg），（3）PIAQ（0.035 mg·kg），（4）盐酸雷尼替丁（50 mg·kg），（5）生理盐水（0.9%），或（6）不干预（假手术）。PIAT和PIAQ均能保护胃黏膜，防止出血性病变、水肿和黏液流失。在肝脏和胰腺细胞中未观察到PIAT或PIAQ的组织学毒性作用。我们的结果表明，绒毛刺桐种子中的蛋白质分离物具有潜在的胃保护作用，使这些化合物成为预防胃溃疡的天然候选物。
