Lőrincz Kende, Haluszka Dóra, Kiss Norbert, Gyöngyösi Nóra, Bánvölgyi András, Szipőcs Róbert, Wikonkál Norbert M
Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 41 Mária Street, Budapest, 1085, Hungary.
Institute for Solid State Physics and Optics of Wigner RCP, Budapest, Hungary.
Arch Dermatol Res. 2017 Apr;309(3):209-215. doi: 10.1007/s00403-017-1715-6. Epub 2017 Feb 8.
Obesity is a risk factor for several cardiovascular and metabolic diseases. Its influence on the skin is less obvious, yet certain negative effects of adipose tissue inflammation on the dermis have been suggested. Excess weight is closely associated with sedentary behavior, so any increase in physical activity is considered beneficial against obesity. To investigate the effects of obesity and physical exercise on the skin, we established a mouse model in which mice were kept either on a high-fat diet or received standard chow. After the two groups achieved a significant weight difference, physical exercise was introduced to both. Animals were given the opportunity to perform voluntary exercise for 40 min daily in a hamster wheel for a period of 8 weeks. We evaluated the status of the dermis at the beginning and at the end of the exercise period by in vivo nonlinear microscopy. Obese mice kept on high-fat diet lost weight steadily after they started to exercise. In the high-fat diet group, we could detect significantly larger adipocytes and a thicker layer of subcutaneous tissue; both changes started to normalize after exercise. Nonlinear microscopy revealed an impaired collagen structure in obese mice that improved considerably after physical activity was introduced. With the ability to detect damage on collagen structure, we set out to address the question whether this process is reversible. With the use of a novel imaging method, we were able to show the reversibility of connective tissue deterioration as a benefit of physical exercise.
肥胖是多种心血管和代谢疾病的风险因素。其对皮肤的影响不太明显，但已有研究表明脂肪组织炎症对真皮有一定的负面影响。超重与久坐行为密切相关，因此任何体力活动的增加都被认为对对抗肥胖有益。为了研究肥胖和体育锻炼对皮肤的影响，我们建立了一个小鼠模型，将小鼠分为两组，一组喂食高脂饮食，另一组喂食标准饲料。在两组小鼠体重出现显著差异后，对两组小鼠都引入体育锻炼。让动物们每天有机会在仓鼠轮中进行40分钟的自愿运动，持续8周。我们通过体内非线性显微镜在运动期开始和结束时评估真皮的状态。高脂饮食喂养的肥胖小鼠开始运动后体重稳步下降。在高脂饮食组中，我们可以检测到明显更大的脂肪细胞和更厚的皮下组织层；运动后这两种变化开始恢复正常。非线性显微镜显示肥胖小鼠的胶原结构受损，在引入体育活动后有显著改善。鉴于能够检测胶原结构的损伤，我们着手研究这个过程是否可逆。通过使用一种新的成像方法，我们能够证明结缔组织退化的可逆性是体育锻炼的一个益处。
