Lou Neng-Jun, Ni Yi-Hong, Jia Hong-Ying, Deng Jing-Ti, Jiang Lu, Zheng Feng-Jie, Sun Ai-Li
The Second Hospital of Shandong University, 247 Beiyuan Street, Ji'nan, Shandong 250033, China.
Department of Biochemistry, School of Medicine of Shandong University, Shandong, China.
J Diabetes Res. 2017;2017:3918681. doi: 10.1155/2017/3918681. Epub 2017 Jan 15.
This study was designed to investigate the changes of urinary microvesicle-bound uromodulin and total urinary uromodulin levels in human urine and the correlations with the severity of diabetic kidney disease (DKD). 31 healthy subjects without diabetes and 100 patients with type 2 diabetes mellitus (T2DM) were included in this study. The patients with T2DM were divided into three groups based on the urinary albumin/creatinine ratio (UACR): normoalbuminuria group (DM, = 46); microalbuminuria group (DN1, = 32); and macroalbuminuria group (DN2, = 22). We use a specific monoclonal antibody AD-1 to capture the urinary microvesicles. Urinary microvesicle-bound uromodulin and total urinary uromodulin levels were determined by enzyme-linked immunosorbent assay (ELISA). Our results showed that the levels of urinary microvesicle-bound uromodulin in DN1 and DN2 groups were significantly higher than those in control group and DM group ( < 0.01). Multiple stepwise linear regression analysis showed that UACR was independent determinant for urinary microvesicle-bound uromodulin ( < 0.05) but not for total urinary uromodulin. These findings suggest that the levels of urinary microvesicle-bound uromodulin are associated with the severity of DKD. The uromodulin in urinary microvesicles may be a specific marker of DKD and potentially may be used to predict the onset and/or monitor the progression of DKD.
本研究旨在探讨人尿中尿微泡结合型尿调节蛋白和总尿调节蛋白水平的变化及其与糖尿病肾病（DKD）严重程度的相关性。本研究纳入了31名无糖尿病的健康受试者和100名2型糖尿病（T2DM）患者。T2DM患者根据尿白蛋白/肌酐比值（UACR）分为三组：正常白蛋白尿组（DM，n = 46）；微量白蛋白尿组（DN1，n = 32）；和大量白蛋白尿组（DN2，n = 22）。我们使用特异性单克隆抗体AD-1捕获尿微泡。通过酶联免疫吸附测定（ELISA）测定尿微泡结合型尿调节蛋白和总尿调节蛋白水平。我们的结果显示，DN1组和DN2组的尿微泡结合型尿调节蛋白水平显著高于对照组和DM组（P < 0.01）。多元逐步线性回归分析显示，UACR是尿微泡结合型尿调节蛋白的独立决定因素（P < 0.05），但不是总尿调节蛋白的独立决定因素。这些发现表明，尿微泡结合型尿调节蛋白水平与DKD的严重程度相关。尿微泡中的尿调节蛋白可能是DKD的特异性标志物， potentially may be used to predict the onset and/or monitor the progression of DKD.（原文此处表述有误，正确表述可能是“and potentially may be used to predict the onset and/or monitor the progression of DKD.”，翻译为“并且有可能用于预测DKD的发病和/或监测其进展”） 可能可用于预测DKD 的发病和/或监测其进展。
