Cam Y, Boukari A, Ruch J V
Institut de Biologie Médicale, INSERM CJF No. 88-08, Faculté de Médecine, Strasbourg, France.
Arch Oral Biol. 1989;34(3):153-9. doi: 10.1016/0003-9969(89)90002-2.
The effects of transferrin on the proliferation kinetics of these cells from day-14 lower first molars, cultured for 2-6 days in a chemically defined medium supplemented with 5 and 50 micrograms/ml of human diferric transferrin, were studied. Transferrin stimulated the mitotic and [3H]-thymidine labelling indices. These data were correlated with immunolocalization of the transferrin receptor using indirect immunofluorescence and specific monoclonal antibodies. The presence of specific transferrin receptors in pre-odontoblasts and pre-ameloblasts, and in ameloblasts of older teeth (day-18 to day-21), was also assessed by indirect immunofluorescence and binding experiments using iodinated transferrin.
