Drouin-Chartier Jean-Philippe, Tremblay André J, Hogue Jean-Charles, Leclerc Myriam, Labonté Marie-Ève, Marin Johanne, Lamarche Benoît, Couture Patrick
Institute of Nutrition and Functional Foods, Laval University, Quebec City, Canada.
CHUQ Research Center, Laval University, Quebec City, Canada.
Metabolism. 2017 Mar;68:163-172. doi: 10.1016/j.metabol.2016.11.019. Epub 2016 Dec 27.
The pro-inflammatory state and elevated plasma levels of post-prandial triglycerides (TG) are associated with increased cardiovascular disease risk. Recent studies suggested that the increase in the production rate of post-prandial lipoproteins observed in patients with insulin resistance (IR) may be caused, at least in part, by the dysregulation of intestinal insulin sensitivity triggered by inflammation.
The objective of the present study was to evaluate the association between IR, plasma C-reactive protein (CRP) levels and the kinetics of TG-rich lipoprotein (TRL) containing apolipoprotein (apo) B-48 in a large sample of insulin sensitive (IS) and IR men.
The in vivo kinetics of TRL apoB-48 were measured in 151 men following a primed-constant infusion of l-[5,5,5-D]leucine. IR subjects (n=91) were characterized by fasting TG levels ≥1.5mmol/L and an index of homeostasis model assessment of IR (HOMA-IR)≥2.5 or type 2 diabetes, while IS subjects (n=24) were characterized by an HOMA-IR index <2.5 and TG levels <1.5mmol/L.
IR subjects had higher TRL apoB-48 production rate (+202%; P<0.0001) and CRP levels (+51%; P=0.01) than IS subjects. TRL apoB-48 production rate and CRP levels were inversely correlated in IR subjects (r=-0.32; P=0.002). IR subjects with CRP levels above the median (2.20mg/L) had lower TRL apoB-48 production rate than IR subjects with CRP levels below the median (Δ=-24%; P<0.05).
Our results confirm that IR is associated with increased TRL apoB-48 secretion and suggest that a higher inflammatory status is associated with decreased TRL apoB-48 secretion among IR subjects.
促炎状态和餐后甘油三酯（TG）血浆水平升高与心血管疾病风险增加相关。最近的研究表明，胰岛素抵抗（IR）患者中观察到的餐后脂蛋白生成率增加可能至少部分是由炎症引发的肠道胰岛素敏感性失调所致。
本研究的目的是在大量胰岛素敏感（IS）和IR男性样本中评估IR、血浆C反应蛋白（CRP）水平与含载脂蛋白（apo）B-48的富含TG脂蛋白（TRL）动力学之间的关联。
在151名男性中，通过一次静脉注射和持续输注l-[5,5,5-D]亮氨酸来测量TRL apoB-48的体内动力学。IR受试者（n = 91）的特征为空腹TG水平≥1.5mmol/L且IR的稳态模型评估指数（HOMA-IR）≥2.5或患有2型糖尿病，而IS受试者（n = 24）的特征为HOMA-IR指数<2.5且TG水平<1.5mmol/L。
IR受试者的TRL apoB-48生成率（+202%；P<0.0001）和CRP水平（+51%；P = 0.01）均高于IS受试者。IR受试者中，TRL apoB-48生成率与CRP水平呈负相关（r = -0.32；P = 0.002）。CRP水平高于中位数（2.20mg/L）的IR受试者的TRL apoB-48生成率低于CRP水平低于中位数的IR受试者（差值=-24%；P<0.05）。
我们的结果证实IR与TRL apoB-48分泌增加相关，并表明在IR受试者中，较高的炎症状态与TRL apoB-48分泌减少相关。
