Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, School of Metallury and Materials Engineering, Chongqing University of Science and Technology, Chongqing, China; Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:198-205. doi: 10.1016/j.msec.2016.12.070. Epub 2016 Dec 15.
Thrombosis formation, restenosis, and delayed endothelium regeneration continue to be a challenge for coronary artery stent therapy. To improve the hemocompatibility of cardiovascular implants and to selectively direct vascular cell behavior, a novel heparin/poly-l-lysine microsphere was developed and immobilized on a dopamine-coated surface. We chose medical grade high nitrogen nickel-free austenitic stainless steel as the stent material since it has better biocompatibility. The stability and structural characteristics of the microspheres changed with the heparin: poly-l-lysine concentration ratio. Antithrombin III binding was significantly enhanced. Furthermore, for plasma coagulation tests, the activated partial thromboplastin time and thrombin time were prolonged and depended on the heparinfunction. The modified exhibited excellent stability and anticoagulant activity, and efficiently accelerated endothelialization and anticoagulation. This work has potential application for the design of coronary artery stent surfaces tailored for vascular cell behavior.
血栓形成、再狭窄和内皮细胞再生延迟仍然是冠状动脉支架治疗的一个挑战。为了改善心血管植入物的血液相容性，并选择性地引导血管细胞行为，开发了一种新型肝素/聚赖氨酸微球，并将其固定在多巴胺涂层表面上。我们选择了医用级高氮无镍奥氏体不锈钢作为支架材料，因为它具有更好的生物相容性。微球的稳定性和结构特性随肝素：聚赖氨酸浓度比而变化。抗凝血酶 III 结合显著增强。此外，对于血浆凝固试验，活化部分凝血活酶时间和凝血酶时间延长，且取决于肝素功能。修饰后的微球表现出优异的稳定性和抗凝活性，并能有效促进内皮化和抗凝作用。这项工作对于设计针对血管细胞行为的冠状动脉支架表面具有潜在的应用价值。
