[Chromosome analysis for determining paternity using the AMBA/R-Chro-H image evaluation system].

A version of the software package AMBA/R-CHRO developed by the Institute of Breeding Research Quedlinburg and by the Institute of Forensic Medicine, Friedrich-Schiller-University Jena, which is suited for the evaluation of banded human chromosomes, is presented. The parameter which are required for paternity diagnostics (absolute length of the chromosomes, absolute and relative length of the heterochromatin block near the centromere) were automatic registrated and calculated. The way of presenting the chromosomes of interest (A 1, C 9, E 16, Y), their grey-value profiles and their schematic representation make understanding, especially for nonexpert clients, easy. The present software package rationalizes work in the cytogenetic laboratory by reducing objectively and personally conditioned error sources. In addition to this, considerable saving in time is reached. The software package for the interactive automatic microscope image analysis primary developed for using in analysis of vegetable chromosomes finds a new range of application in the field of settlement of paternity cases.