长链非编码RNA-DC介导子痫前期蜕膜树突状细胞过度成熟并诱导辅助性T细胞1增加。
Lnc-DC mediates the over-maturation of decidual dendritic cells and induces the increase in Th1 cells in preeclampsia.
作者信息
Zhang Wen, Zhou Yang, Ding YiLing
机构信息
Department of Obstetrics and Gynecology, Second XiangYa Hospital of Central South University, Changsha, Hunan province, China.
出版信息
Am J Reprod Immunol. 2017 Jun;77(6). doi: 10.1111/aji.12647. Epub 2017 Feb 10.
PROBLEM
Preeclampsia is a severe pregnancy-related disease that involves an imbalance of the immune reaction, which is dominated by T helper1 (Th1) cells; however, the mechanism is unclear.
METHOD OF STUDY
We used flow cytometry, RT-PCR and western blotting to detect Th1 cells in the blood and mature dendritic cells, lnc-DC (a long non-coding RNA that is specifically expressed in DCs and can mediate dendritic cell maturation by phosphorylating STAT3) and p-STAT3 (phosphorylated transducer and activator of transcription 3) in the deciduas of normal pregnancies and preeclampsia patients.
RESULTS
We found that the expression of lnc-DC and p-STAT3 was increased in the deciduas of preeclampsia patients. The proportion of Th1 cells and mature dendritic cells was significantly higher in the preeclampsia patients.
CONCLUSION
These results suggested the hypothesis that the overexpression of lnc-DCs induces the over-maturation of decidual dendritic cells in preeclampsia patients and leads to an increase in Th1 cells.
问题
子痫前期是一种与妊娠相关的严重疾病，涉及免疫反应失衡，该免疫反应以辅助性T细胞1（Th1）为主导；然而，其机制尚不清楚。
研究方法
我们使用流式细胞术、逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测正常妊娠和子痫前期患者蜕膜中血液和成熟树突状细胞、lnc-DC（一种在树突状细胞中特异性表达并可通过磷酸化信号转导和转录激活因子3（STAT3）介导树突状细胞成熟的长链非编码RNA）和磷酸化STAT3（p-STAT3）中的Th1细胞。
结果
我们发现子痫前期患者蜕膜中lnc-DC和p-STAT3的表达增加。子痫前期患者中Th1细胞和成熟树突状细胞的比例显著更高。
结论
这些结果提示了一种假说，即lnc-DC的过表达诱导子痫前期患者蜕膜树突状细胞过度成熟，并导致Th1细胞增加。
Zotero 插件