突触浆中乙酰辅酶A在神经末梢乙酰胆碱合成中的调节功能的证据。
Evidence for the regulatory function of synaptoplasmic acetyl-CoA in acetylcholine synthesis in nerve endings.
作者信息
Bielarczyk H, Szutowicz A
机构信息
Department of Clinical Biochemistry, Medical Academy, Gdańsk, Poland.
出版信息
Biochem J. 1989 Aug 15;262(1):377-80. doi: 10.1042/bj2620377.
Abstract
Isolated synaptosomes maintained a relatively stable level of acetyl-CoA during their incubation in the presence of 30 mM-KCl. Addition of Ca2+ resulted in inhibition of pyruvate oxidation and slight activation of acetylcholine synthesis. The cation decreased acetyl-CoA in intrasynaptosomal mitochondria, but did not alter its content in synaptoplasm. Verapamil did not affect pyruvate oxidation, but decreased acetyl-CoA in synaptoplasm and inhibited acetylcholine synthesis in synaptosomes. It indicates that Ca2+ might regulate acetylcholine synthesis through changes in the direct transfer of acetyl-CoA from mitochondria to synaptoplasm.
摘要
在含有30 mM - KCl的条件下孵育时，分离的突触体维持着相对稳定水平的乙酰辅酶A。添加Ca2+会导致丙酮酸氧化受到抑制，乙酰胆碱合成略有激活。该阳离子降低了突触体内线粒体中的乙酰辅酶A，但并未改变其在突触质中的含量。维拉帕米不影响丙酮酸氧化，但降低了突触质中的乙酰辅酶A，并抑制了突触体中的乙酰胆碱合成。这表明Ca2+可能通过改变乙酰辅酶A从线粒体到突触质的直接转运来调节乙酰胆碱的合成。
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本文引用的文献
1
The role of citrate derived from glucose in the acetylcholine synthesis in rat brain synaptosomes.
Int J Biochem. 1981;13(8):887-92. doi: 10.1016/0020-711x(81)90014-8.
2
Acetyl coenzyme A and acetylcholine in slices of rat caudate nuclei incubated in the presence of metabolic inhibitors.
J Biol Chem. 1981 May 25;256(10):4919-23.
3
The effect of [Ca2+] and [H+] on the functional recovery of rat brain synaptosomes from anoxic insult in vitro.
Biochem J. 1983 May 15;212(2):289-95. doi: 10.1042/bj2120289.
4
Utilization of citrate, acetylcarnitine, acetate, pyruvate and glucose for the synthesis of acetylcholine in rat brain slices.
J Neurochem. 1981 Apr;36(4):1323-30. doi: 10.1111/j.1471-4159.1981.tb00569.x.
5
Acetylcholine and oxidative metabolism in septum and hippocampus in vitro.
J Biol Chem. 1983 Jan 25;258(2):1142-5.
6
Problems in the organization and control of acetylcholine synthesis in brain neurons.
Prog Biophys Mol Biol. 1984;44(1):1-46. doi: 10.1016/0079-6107(84)90011-7.
7
Selective inhibition of Na+-induced Ca2+ release from heart mitochondria by diltiazem and certain other Ca2+ antagonist drugs.
J Biol Chem. 1982 Jun 10;257(11):6000-2.
8
(14C) acetylcholine synthesis by cortex slices of rat brain.
J Neurochem. 1968 Dec;15(12):1391-405. doi: 10.1111/j.1471-4159.1968.tb05921.x.
9
Preferential release of newly synthesized 3 H-acetylcholine from rat cerebral cortex slices in vitro.
Br J Pharmacol. 1973 Jan;47(1):97-108. doi: 10.1111/j.1476-5381.1973.tb08162.x.
10
Kinetics of choline acetyltransferases (EC 2.3.1.6) from human and other mammalian central and peripheral nervous tissues.
J Neurochem. 1973 Feb;20(2):297-307. doi: 10.1111/j.1471-4159.1973.tb12129.x.
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