Szutowicz A, Tomaszewicz M, Jankowska A, Kisielevski Y
Department of Clinical Biochemistry, Gdańsk School of Medicine, Poland.
Neuroreport. 1994 Dec 20;5(18):2421-4. doi: 10.1097/00001756-199412000-00004.
Streprozotocin diabetes and extracerebral insulin affect acetyl-CoA and acetylcholine metabolism in the brain. In the present study we have shown that pyruvate utilization, acetyl-CoA content and ACh synthesis in nerve terminals from diabetic rats were 45, 30 and 50%, respectively, higher than that in healthy animals. Treatment with insulin normalized pyruvate utilization and acetylcholine synthesis but did not decrease the acetyl-CoA level. 3-Hydroxybutyrate did not affect acetyl-CoA and acetylcholine metabolism in control rats. However, in diabetic animals, 3-hydroxybutyrate significantly increased supply of acetyl-CoA for acetylcholine synthesis. These data provide evidence that increased provision of acetyl-CoA is prerequisite for activation of acetylcholine synthesis in diabetic brain.
链脲佐菌素糖尿病和脑外胰岛素影响大脑中的乙酰辅酶A和乙酰胆碱代谢。在本研究中，我们发现糖尿病大鼠神经末梢中的丙酮酸利用、乙酰辅酶A含量和乙酰胆碱合成分别比健康动物高45%、30%和50%。胰岛素治疗使丙酮酸利用和乙酰胆碱合成恢复正常，但并未降低乙酰辅酶A水平。3-羟基丁酸对对照大鼠的乙酰辅酶A和乙酰胆碱代谢没有影响。然而，在糖尿病动物中，3-羟基丁酸显著增加了用于乙酰胆碱合成的乙酰辅酶A供应。这些数据证明，增加乙酰辅酶A的供应是糖尿病大脑中乙酰胆碱合成激活的先决条件。
