Miller W C, Gorski J, Oscai L B, Palmer W K
Dept. of Physical Education, Indiana University, Bloomington 47405.
Biochem Biophys Res Commun. 1989 Oct 31;164(2):615-9. doi: 10.1016/0006-291x(89)91504-0.
Epinephrine was used to activate the heparin non-releasable lipoprotein lipase (LPL) in the 3 skeletal muscle fiber types of the perfused rat hindlimb. Following a 9 min washout of the capillary-bound lipoprotein lipase, the hindquarter of the rat was perfused with a buffer containing 10 nM of epinephrine. Activity of the residual LPL in soleus, red vastus lateralis, and white vastus lateralis muscles increased 75%, 96%, and 102% respectively, following epinephrine perfusion. These results suggest that skeletal muscle LPL is under hormonal control possibly through protein phosphorylation by cyclic AMP dependent protein kinase.
肾上腺素被用于激活灌注大鼠后肢的3种骨骼肌纤维类型中的肝素不可释放脂蛋白脂肪酶（LPL）。在对毛细血管结合的脂蛋白脂肪酶进行9分钟洗脱后，用含有10 nM肾上腺素的缓冲液灌注大鼠的后肢。肾上腺素灌注后，比目鱼肌、红色股外侧肌和白色股外侧肌中残余LPL的活性分别增加了75%、96%和102%。这些结果表明，骨骼肌LPL可能受激素控制，可能是通过环磷酸腺苷依赖性蛋白激酶的蛋白质磷酸化作用。
