在磷脂酰胆碱三元混合物中，蜂毒肽与二肉豆蔻酰磷脂酰胆碱之间的区域匹配疏水相互作用。

A region-matched hydrophobic interaction between melittin and dimyristoylphosphatidylcholine in a ternary mixture of phosphatidylcholines.

作者信息

Ohki K

机构信息

Department of Applied Physics, School of Engineering, Nagoya University, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Oct 31;164(2):850-6. doi: 10.1016/0006-291x(89)91536-2.

DOI:10.1016/0006-291x(89)91536-2

PMID:2818591

Abstract

Interaction of melittin with phosphatidylcholine molecules in pure vesicles, binary mixtures and a ternary mixture of dimyristoylphosphatidylcholine IDMPC), dipalmitoylphosphatidylcholine (DPPC) and distearoylphosphatidylcholine (DSPC) was investigated by differential scanning calorimetry. Melittin binds preferentially with DMPC, and results in segregation of DMPC in binary mixtures of DMPC/DPPC and DMPC/DSPC and in a ternary mixture of DMPC/DPPC/DSPC. The results indicate that the hydrophobic part of peptide interacts preferentially with the phospholipid which has the same size of hydrophobic region or fatty acyl chains.

摘要

通过差示扫描量热法研究了蜂毒肽与纯囊泡、二肉豆蔻酰磷脂酰胆碱（DMPC）、二棕榈酰磷脂酰胆碱（DPPC）和二硬脂酰磷脂酰胆碱（DSPC）的二元混合物以及三元混合物中磷脂酰胆碱分子的相互作用。蜂毒肽优先与DMPC结合，并导致DMPC在DMPC/DPPC和DMPC/DSPC的二元混合物以及DMPC/DPPC/DSPC的三元混合物中发生分离。结果表明，肽的疏水部分优先与具有相同疏水区域大小或脂肪酰链的磷脂相互作用。