Nedospasov S A, Turetskaia R L, Mett V A, Shakhov A N
Bioorg Khim. 1989 Jul;15(7):990-3.
We have developed a technique for restriction nuclease sites mapping in genomic DNA cloned into phage lambda vectors. Synthetic oligonucleotides homologous to the vector sequences and adjacent to the cloning site were used as hybridisation probes for indirect end labelling procedure. In addition, a number of oligonucleotides homologous to the sequences of tumour necrosis factor genes were used for mapping from the internal sites. As a result, a map of 35 kb genomic region on the chromosome 17 inside major histocompatibility complex and surrounding mouse tumour necrosis factor genes was constructed.
我们已经开发出一种技术，用于绘制克隆到噬菌体λ载体中的基因组DNA中的限制性核酸酶位点图谱。与载体序列同源且与克隆位点相邻的合成寡核苷酸被用作间接末端标记程序的杂交探针。此外，一些与肿瘤坏死因子基因序列同源的寡核苷酸被用于从内部位点进行图谱绘制。结果，构建了主要组织相容性复合体内部17号染色体上35kb基因组区域以及周围小鼠肿瘤坏死因子基因的图谱。
